Biosynthesis and properties of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646

Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1002/jctb.5763

Ключевые слова: 3-hydroxy-4-methylvalerate, physicochemical properties, polyhydroxyalkanoates, synthesis

Аннотация: BACKGROUND: Polyhydroxyalkanoates (PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L−1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry

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Издание

Журнал: Journal of Chemical Technology and Biotechnology

Издатель: John Wiley and Sons Ltd

Авторы

  • Volova Tatiana (Siberian Fed Univ, Krasnoyarsk, Russia; RAS, SB, Krasnoyarsk Sci Ctr, Fed Res Ctr,Inst Biophys, Krasnoyarsk, Russia)
  • Menshikova Olga (RAS, SB, Krasnoyarsk Sci Ctr, Fed Res Ctr,Inst Biophys, Krasnoyarsk, Russia)
  • Zhila Natalia (Siberian Fed Univ, Krasnoyarsk, Russia; RAS, SB, Krasnoyarsk Sci Ctr, Fed Res Ctr,Inst Biophys, Krasnoyarsk, Russia)
  • Vasiliev Alexander (Siberian Fed Univ, Krasnoyarsk, Russia; RAS, SB, LV Kirenskii Inst Phys, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia)
  • Kiselev Evgeniy (Siberian Fed Univ, Krasnoyarsk, Russia; RAS, SB, Krasnoyarsk Sci Ctr, Fed Res Ctr,Inst Biophys, Krasnoyarsk, Russia)
  • Peterson Ivan (RAS, SB, Inst Chem & Chem Technol, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia)
  • Shishatskaya Ekaterina (Siberian Fed Univ, Krasnoyarsk, Russia; RAS, SB, Krasnoyarsk Sci Ctr, Fed Res Ctr,Inst Biophys, Krasnoyarsk, Russia)
  • Thomas Sabu (Siberian Fed Univ, Krasnoyarsk, Russia; Mahatam Gandhi Univ, Int & Inter Univ Ctr Nanosci & Nanotechnol, Kottyam, India)

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