The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems

Тип публикации: статья из журнала

Год издания: 2001

Идентификатор DOI: 10.1016/S0045-6535(00)00177-6

Ключевые слова: bioluminescence; bacterial luciferase; alcohol dehydrogenase; trypsin; water toxicity; blooming; pollution

Аннотация: A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductaseluciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Издание

Журнал: CHEMOSPHERE

Выпуск журнала: Vol. 42, Is. 8

Номера страниц: 909-915

ISSN журнала: 00456535

Место издания: OXFORD

Издатель: PERGAMON-ELSEVIER SCIENCE LTD

Авторы

  • Kratasyuk V.A. (Krasnoyarsk State University)
  • Esimbekova E.N. (Institute of Biophysics,Siberian Branch,Russian Academy of Sciences)
  • Gladyshev M.I. (Institute of Biophysics,Siberian Branch,Russian Academy of Sciences)
  • Khromichek E.B. (Institute of Biophysics,Siberian Branch,Russian Academy of Sciences)
  • Kuznetsov A.M. (Institute of Biophysics,Siberian Branch,Russian Academy of Sciences)
  • Ivanova E.A. (Krasnoyarsk State Agricultural University)

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