Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1038/s41598-017-02632-0

Аннотация: MicroRNAs (miRNAs) constitute a class of small noncoding RNAs that plays an important role in the post-transcriptional regulation of gene expression. Much evidence has demonstrated that miRNAs are involved in regulating the human and mouse pluripotency. Nevertheless, to our knowledge, miRNAs in the pluripotent stem cells of one of the most commonly used model organisms - the Rattus norvegicus have not been studied. In the present study, we performed deep sequencing of small RNA molecules in the embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells of laboratory rats. Bioinformatics analysis revealed 674 known miRNAs and 394 novel miRNA candidates in all of the samples. Expression of known pluripotency-associated miRNAs, such as the miR-290-295 and miR-183-96-182 clusters as well as members of the miR-200 family, was detected in rat pluripotent stem cells. Analysis of the targets of differentially expressed known and novel miRNAs showed their involvement in the regulation of pluripotency and the reprogramming process in rats. Bioinformatics and systems biology approaches identified potential pathways that are regulated by these miRNAs. This study contributes to our understanding of miRNAs in the regulation of pluripotency and cell reprogramming in the laboratory rat. © The Author(s) 2017.

Журнал: Scientific Reports

Выпуск журнала: Vol. 7, Is. 1

Номера страниц: 2787

ISSN журнала: 20452322

Издатель: Nature Publishing Group

• Sherstyuk V.V. (Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10 Lavrentyeva Ave., Novosibirsk, Russian Federation, Siberian Federal Biomedical Research Center, Ministry of Healthcare of the Russian Federation, 15 Rechkunovskaya St., Novosibirsk, Russian Federation, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentyeva Ave., Novosibirsk, Russian Federation, Novosibirsk State University, 2 Pirogova St., Novosibirsk, Russian Federation)
• Medvedev S.P. (Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10 Lavrentyeva Ave., Novosibirsk, Russian Federation, Siberian Federal Biomedical Research Center, Ministry of Healthcare of the Russian Federation, 15 Rechkunovskaya St., Novosibirsk, Russian Federation, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentyeva Ave., Novosibirsk, Russian Federation, Novosibirsk State University, 2 Pirogova St., Novosibirsk, Russian Federation)
• Elisaphenko E.A. (Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10 Lavrentyeva Ave., Novosibirsk, Russian Federation, Siberian Federal Biomedical Research Center, Ministry of Healthcare of the Russian Federation, 15 Rechkunovskaya St., Novosibirsk, Russian Federation, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentyeva Ave., Novosibirsk, Russian Federation)
• Vaskova E.A. (Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10 Lavrentyeva Ave., Novosibirsk, Russian Federation, Siberian Federal Biomedical Research Center, Ministry of Healthcare of the Russian Federation, 15 Rechkunovskaya St., Novosibirsk, Russian Federation, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentyeva Ave., Novosibirsk, Russian Federation)
• Ri M.T. (AcademGene LLC, 6 Lavrentyeva Ave., Novosibirsk, Russian Federation, St. Laurent Institute, 317 New Boston St., Woburn, MA, United States)
• Vyatkin Y.V. (Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10 Lavrentyeva Ave., Novosibirsk, Russian Federation, Novosibirsk State University, 2 Pirogova St., Novosibirsk, Russian Federation, AcademGene LLC, 6 Lavrentyeva Ave., Novosibirsk, Russian Federation, St. Laurent Institute, 317 New Boston St., Woburn, MA, United States)
• Saik O.V. (Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10 Lavrentyeva Ave., Novosibirsk, Russian Federation)
• Shtokalo D.N. (Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10 Lavrentyeva Ave., Novosibirsk, Russian Federation, AcademGene LLC, 6 Lavrentyeva Ave., Novosibirsk, Russian Federation, St. Laurent Institute, 317 New Boston St., Woburn, MA, United States, A.P. Ershov Institute of Informatics Systems, 6 Lavrentyeva Ave., Novosibirsk, Russian Federation)
• Pokushalov E.A. (Siberian Federal Biomedical Research Center, Ministry of Healthcare of the Russian Federation, 15 Rechkunovskaya St., Novosibirsk, Russian Federation)
• Zakian S.M. (Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10 Lavrentyeva Ave., Novosibirsk, Russian Federation, Siberian Federal Biomedical Research Center, Ministry of Healthcare of the Russian Federation, 15 Rechkunovskaya St., Novosibirsk, Russian Federation, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentyeva Ave., Novosibirsk, Russian Federation, Novosibirsk State University, 2 Pirogova St., Novosibirsk, Russian Federation)

• Scopus