Тип публикации: статья из журнала
Год издания: 2015
Идентификатор DOI: 10.1134/S0026893315050039
Ключевые слова: single nucleotide polymorphisms, melanoma, MC1R receptor, bioluminescent assay
Аннотация: Several polymorphisms in the melanocortin-1 receptor gene (MC1R) have been associated with melanoma risk. In particular, rs1805007, rs1805008, and rs1805009 mutations, which result in R151C, R160W, and D294H amino acid substitutions, respectively, and are associated with the phenotype of red-hair mutations, have also been connected with melanoma and nonmelanoma skin cancer risks. This work describes a method of detecting these polymorphisms using primer extension with subsequent dual bioluminescent assay. Model plasmids carrying polymorphic MC1R fragments, as well as several clinical DNA samples, were tested using the proposed technique. The results agreed well with those obtained by Sanger sequencing.
Издание
Журнал: MOLECULAR BIOLOGY
Выпуск журнала: Vol. 49, Is. 6
Номера страниц: 852-857
ISSN журнала: 00268933
Место издания: NEW YORK
Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER
Персоны
- Bashmakova E.E. (Institute of Biophysics,Siberian Branch of the Russian Academy of Sciences)
- Frank L.A. (Institute of Biophysics,Siberian Branch of the Russian Academy of Sciences)
- Krasitskaya V.V. (Institute of Biophysics,Siberian Branch of the Russian Academy of Sciences)
- Bondar A.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch of the Russian Academy of Sciences)
- Kozlova A.V. (Voino-Yasenetskii Krasnoyarsk State Medical University)
- Ruksha T.G. (Voino-Yasenetskii Krasnoyarsk State Medical University)
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