Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2019

Идентификатор DOI: 10.3390/plants8110439

Ключевые слова: Cytoplasmic male sterility (CMS), Mitochondrial genome, Next generation sequencing (NGS), Reorganizations, Sunflower

Аннотация: This study provides insights into the flexibility of the mitochondrial genome in sunflower (Helianthus annuus L.) as well as into the causes of ANN2-type cytoplasmic male sterility (CMS). De novo assembly of the mitochondrial genome of male-sterile HA89(ANN2) sunflower line was performed using high-throughput sequencing technologies. Analysis of CMS ANN2 mitochondrial DNA sequence revealed the following reorganization events: Twelve rearrangements, seven insertions, and nine deletions. Comparisons of coding sequences from the male-sterile line with the male-fertile line identified a deletion of orf777 and seven new transcriptionally active open reading frames (ORFs): Orf324, orf327, orf345, orf558, orf891, orf933, orf1197. Three of these ORFs represent chimeric genes involving atp6 (orf1197), cox2 (orf558), and nad6 (orf891). In addition, orf558, orf891, orf1197, as well as orf933, encode proteins containing membrane domain(s), making them the most likely candidate genes for CMS development in ANN2. Although the investigated CMS phenotype may be caused by simultaneous action of several candidate genes, we assume that orf1197 plays a major role in developing male sterility in ANN2. Comparative analysis of mitogenome organization in sunflower lines representing different CMS sources also allowed identification of reorganization hot spots in the mitochondrial genome of sunflower. © 2019, MDPI AG. All rights reserved.

Журнал: Plants

Выпуск журнала: Vol. 8, Is. 11

Номера страниц: 439

ISSN журнала: 22237747

Издатель: MDPI AG

• Makarenko Maksim S. (Southern Fed Univ, Dept Genet, Rostov Na Donu 344006, Russia; Inst Informat Transmiss Problems, Moscow 127051, Russia)
• Usatov Alexander (Southern Fed Univ, Dept Genet, Rostov Na Donu 344006, Russia)
• Tatarinova Tatiana (Inst Informat Transmiss Problems, Moscow 127051, Russia; Univ La Verne, Dept Biol, La Verne, CA 91750 USA; Vavilov Inst Gen Genet, Moscow 119333, Russia; Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia)
• Azarin Kirill (Southern Fed Univ, Dept Genet, Rostov Na Donu 344006, Russia)
• Logacheva Maria D. (Inst Informat Transmiss Problems, Moscow 127051, Russia; Skolkovo Inst Sci & Technol, Moscow 121205, Russia)
• Gavrilova Vera A. (NI Vavilov All Russian Inst Plant Genet Resource, St Petersburg 190121, Russia)
• Kornienko Igor (Southern Fed Univ, Dept Genet, Rostov Na Donu 344006, Russia; Russian Acad Sci, Southern Sci Ctr, Rostov Na Donu 344006, Russia)
• Horn Renate (Univ Rostock, Inst Biol Sci, Plant Genet, D-18059 Rostock, GermanyArticle)

• Scopus
• Web of Science Core Collection
• Ядро РИНЦ (eLIBRARY.RU)