Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.3390/bioengineering9020061

Ключевые слова: bioassay, gold nanoparticles, hormesis, luminous marine bacteria, reactive oxygen species, tritium

Аннотация: Our current study aimed to adapt a bioluminescent bacteria-based bioassay to monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low-concentration (≤10−3 g/L) bioeffects of AuNPs were studied. Bioluminescence intensity was used as an indicator of physiological activity in bacteria. Two additional methods were used: reactive oxygen species (ROS) content was estimated with a chemiluminescent luminol method, and bacterial size was monitored using electron microscopy. The bacterial bioluminescent response to AuNPs corresponded to the “hormesis” model and involved time-dependent bioluminescence activation, as well as a pro-nounced increase in the number of enlarged bacteria. We found negative correlations between the time courses of bioluminescence and the ROS content in bacterial suspensions, demonstrating the relationship between bioluminescence activation and bacterial ROS consumption. The combined effects of AuNPs and a beta-emitting radionuclide, tritium, revealed suppression of bacterial biolu-minescent activity (as compared to their individual effects) and a reduced percentage of enlarged bacteria. Therefore, we demonstrated that our bacteria-based bioluminescence assay is an appropriate tool to study the bioeffects of AuNPs; the bioeffects can be further classified within a unified framework for rapid bioassessment. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Журнал: Bioengineering

Выпуск журнала: Vol. 9, Is. 2

Номера страниц: 61

ISSN журнала: 23065354

Издатель: MDPI

• Yehia M.R. (Biophysics Department, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)
• Smolyarova T.E. (Institute of Physics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, 660036, Russian Federation)
• Shabanov A.V. (Institute of Physics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, 660036, Russian Federation)
• Sushko E.S. (Institute of Physics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, 660036, Russian Federation, Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, 660036, Russian Federation)
• Badun G.A. (Department of Chemistry, Moscow State University, Moscow, 119991, Russian Federation)
• Kudryasheva N.S. (Biophysics Department, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation, Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, 660036, Russian Federation)

• Scopus
• Web of Science Core Collection
• Ядро РИНЦ (eLIBRARY.RU)