Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1134/S106816202006014X

Ключевые слова: Bioluminescence assay, Hybrid protein, igG, proZZ, Са<sup>2+</sup>-regulated photoprotein obelin

Аннотация: The gene of the hybrid protein that encodes the double synthetic fragment proZZ of the immunoglobulin-binding domain of protein A of Staphylococcus aureus and apo-obelin joined by a short linker has been cloned. The corresponding hybrid protein has been obtained by expression in Esсheriсhia coli cells. The protein activated with a substrate (coelenterazine) possesses the bioluminescent Са²⁺-dependent activity of the photoprotein close to that of recombinant wild-type obelin, and the immunoglobulin-binding ability of protein A. It has been shown that the hybrid can be used as a highly sensitive label to detect antibodies and estimate their affinity and interaction with recombinant proteins, as well as in investigations of other kinds.

Журнал: Russian Journal of Bioorganic Chemistry

Выпуск журнала: Т. 46, № 6

Номера страниц: 1004-1010

ISSN журнала: 10681620

Место издания: Москва

Издатель: Pleiades Publishing, Ltd. (Плеадес Паблишинг, Лтд)

• Krasitskaya V.V. (Institute of Biophysics,Federal Research Center,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
• Bashmakova E.E. (Institute of Biophysics,Federal Research Center,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
• Kudryavtsev A.N. (Institute of Biophysics,Federal Research Center,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
• Frank L.A. (Institute of Biophysics,Federal Research Center,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
• Vorobjeva M.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
• Shatunova E.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)

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