Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.1016/j.bbrc.2022.07.079

Ключевые слова: bioluminescence, coelenterazine, ctenophores, photoprotein, quantum yield, specific activity

Аннотация: The bright bioluminescence of ctenophores inhabiting the oceans worldwide is caused by light-sensitive Ca2+-regulated photoproteins. By now, the cDNAs encoding photoproteins from the four different ctenophore species have been cloned and the recombinant proteins have been characterized to some extent. In this work, we report on the specific activity and the quantum yield of bioluminescence reaction as well as the absorbance characteristics of high-purity recombinant berovin. To determine those, we applied the amino acid composition analysis to accurately measure berovin concentration and the recombinant aequorin as a light standard to convert relative light units to quanta. The extinction coefficient of 1% berovin solution at 435 nm was found to be 1.82. The one can be employed to precisely determine the protein concentration of active photoproteins from other ctenophore species. The specific activity and the bioluminescence quantum yield were respectively found to be 1.98 × 1015 quanta/mg and 0.083. These values appeared to be several times lower than those of the cnidarian photoproteins, which is obviously due to differences in amino acid environments of the substrate in active sites of these photoproteins. © 2022 Elsevier Inc.

Журнал: Biochemical and Biophysical Research Communications

Выпуск журнала: Vol. 624

Номера страниц: 23-27

ISSN журнала: 0006291X

Издатель: Elsevier B.V.

• Burakova L.P. (Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation)
• Kolmakova A.A. (Analytical Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation)
• Vysotski E.S. (Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation)

• Scopus