Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.1134/S1021443722030116

Ключевые слова: cfi integral parameters, chlorophyll fluorescence induction, ontogenesis of plant leaves, raphanus sativus var. radicula pers, triticum aestivum l

Аннотация: Abstract: Possible use of integral characteristics of slow stage of chlorophyll fluorescence induction (CFI) for monitoring of physiological state of plants in phytocenoses was studied in the leaves of wheat Triticum aestivum L. and radish Raphanus sativus var. radicula Pers., cv. Virovskii belyi, grown in artificial-light culture. Investigation concerned the behavior of Т0.5 (half-time of fluorescence intensity decrease from its peak to a steady-state level), Rfd (fluorescence decrease ratio also known as vitality index), parameter (Formula presented.) (maximum possible average rate of induction transient), and (Formula presented.) (average efficiency of PSA in the induction period) during leaf ontogenesis. Plant material was the discs cut from uniformly illuminated wheat leaves of different age from the top (sixth) storey of plants and the disks from the second (in the order of emergence) true leaves of radish at the age from 3 to 24 days. The plants were grown in controlled-climate chambers in hydroponic culture on claydite at irradiance of 100 W/m2 of photosynthetically active radiation (PAR). CFI curves were recorded using a single-beam unit. Values of parameter Т0.5 slightly changed during leaves’ ontogenesis up to the time of senescence (the age of 26 days). Relative changes in the values of CFI parameters (Δ rel., %) at the age from 2 to 24 days were 28% for (Formula presented.) and 30% for Т0.5. For (Formula presented.) and Rfd, the values were 67 and 70%, respectively. Upon more advanced ageing and comparison of parameters for 24- and 26-day-old leaves, more pronounced changes were observed for Т0.5 (by 37.6%) and (Formula presented.) (by 36.0%) and less pronounced for (Formula presented.) (by 23.0%) and Rfd (by 13.0%). Thus, the pattern of parameter Т0.5 behavior observed in the course of wheat leaves’ ontogenesis distinguished it from other fluorescence parameters of CFI slow stage (Rfd, (Formula presented.), and (Formula presented.)); this gives grounds for a potential use of Т0.5 as one of the simplest integral indicators of physiological state and pathophysiological (irreversible) changes occurring in plant leaves within a wide age interval and upon senescence. Unambiguous interpretation of the data obtained by means of parameter Т0.5 under stress conditions for estimation of irreversibility of the observed changes in PSA requires more detailed investigations. © 2022, Pleiades Publishing, Ltd.

Журнал: Russian Journal of Plant Physiology

Выпуск журнала: Vol. 69, Is. 3

Номера страниц: 53

ISSN журнала: 10214437

Издатель: Pleiades journals

• Nesterenko T.V. (Institute of Biophysics, Federal Research Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation)
• Tikhomirov A.A. (Institute of Biophysics, Federal Research Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation, Siberian State University, Krasnoyarsk, Russian Federation)
• Shikhov V.N. (Institute of Biophysics, Federal Research Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation)

• Scopus