New peak broadening parameter for the characterization of separation capability in capillary electrophoresis

Тип публикации: статья из журнала

Год издания: 2015

Идентификатор DOI: 10.1002/jssc.201401109

Ключевые слова: Capillary electrophoresis, Plate number, Peak resolution, Peak width, Virtual migration distance, Electrolyte flows, Migration distance, Peak broadening, Peak widths, Separation condition, Separation efficiency, Theoretical plates, Electrophoretic mobility, anion, electrolyte, formic acid, ion, sulfosalicylic acid, thiocyanate, triethanolamine, Article, diffusion, dispersion, electric potential, electrophoresis, flow rate, hydrodynamics, hydrostatic pressure, priority journal, separation technique

Аннотация: The influence of separation conditions on peak broadening is usually estimated by the number of theoretical plates. Using the data available in literature and experimental data, it is shown that in pressure-assisted capillary electrophoresis the plate number is not directly related to the separation capability of conditions used. The experiments at different electrolyte flow velocities demonstrate that a higher plate number (the best separation efficiency) can be obtained with a lower peak resolution. Since ions are separated by electrophoresis due to the difference in electrophoretic mobilities, the peak width in terms of electrophoretic mobility is suggested as a new peak broadening parameter describing the separation capability of the conditions used. The parameter can be calculated using the tailing factor and the temporal peak width at 5% of the peak height. A simple equation for the resolution calculation is derived using the parameter. The advantage of the peak width in terms of mobility over other parameters is shown. The new parameter is recommended to be used not only in pressure-assisted capillary electrophoresis but also in general capillary electrophoresis when in a number of runs the virtual separative migration distance and separation capability of the conditions used change widely.

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Издание

Журнал: JOURNAL OF SEPARATION SCIENCE

Выпуск журнала: Vol. 38, Is. 4

Номера страниц: 690-696

ISSN журнала: 16159306

Место издания: WEINHEIM

Издатель: WILEY-V C H VERLAG GMBH

Авторы

  • Sursyakova V.V. (Institute of Chemistry and Chemical Technology, Siberian Branch of the Russian Academy of Sciences)
  • Rubaylo A.I. (Siberian Federal University)

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