Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.3390/nano12213843

Ключевые слова: biodegradable polyhydroxyalkanoates (phas), cell cultures, copolymers, films, healing of skin wounds, nanomembranes, properties, wound coverings

Аннотация: From a series of biodegradable natural polymers of polyhydroxyalkanoates (PHAs)—poly-3-hydroxybutyrate (P(3HB) and copolymers containing, in addition to 3HB monomers, monomers of 3-hydroxyvalerate (3HV), 3-hydroxyhexanoate (3HHx), and 4-hydroxybutyrate (4HB), with different ratios of monomers poured—solvent casting films and nanomembranes with oriented and non-oriented ultrathin fibers were obtained by electrostatic molding. With the use of SEM, AFM, and measurement of contact angles and energy characteristics, the surface properties and mechanical and biological properties of the polymer products were studied depending on the method of production and the composition of PHAs. It has been shown in cultures of mouse fibroblasts of the NIH 3T3 line and diploid human embryonic cells of the M22 line that elastic films and nanomembranes composed of P(3HB-co-4HB) copolymers have high biocompatibility and provide adhesion, proliferation and preservation of the high physiological activity of cells for up to 7 days. Polymer films, namely oriented and non-oriented nanomembranes coated with type 1 collagen, are positively evaluated as experimental wound dressings in experiments on laboratory animals with model and surgical skin lesions. The results of planimetric measurements of the dynamics of wound healing and analysis of histological sections showed the regeneration of model skin defects in groups of animals using experimental wound dressings from P(3HB-co-4HB) of all types, but most actively when using non-oriented nanomembranes obtained by electrospinning. The study highlights the importance of nonwoven nanomembranes obtained by electrospinning from degradable low-crystalline copolymers P(3HB-co-4HB) in the effectiveness of the skin wound healing process. © 2022 by the authors.

Журнал: Nanomaterials

Выпуск журнала: Vol. 12, Is. 21

Номера страниц: 3843

ISSN журнала: 20794991

Издатель: MDPI

• Shishatskaya E.I. (Department of Medical Biology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av, Krasnoyarsk, 660041, Russian Federation, Chemistry Engineering Centre, ITMO University, Kronverkskiy Prospekt, 49A, Saint Petersburg, 197101, Russian Federation)
• Dudaev A.E. (Department of Medical Biology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av, Krasnoyarsk, 660041, Russian Federation, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation)
• Volova T.G. (Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation, Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av, Krasnoyarsk, Basic, 660041, Russian Federation)

• Scopus