Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.1038/s42003-021-02510-6

Аннотация: Most of Earth's bacteria have yet to be cultivated. The metabolic and functional potentials of these uncultivated microorganisms thus remain mysterious, and the metagenome-assembled genome (MAG) approach is the most robust method for uncovering these potentials. However, MAGs discovered by conventional metagenomic assembly and binning are usually highly fragmented genomes with heterogeneous sequence contamination. In this study, we combined Illumina and Nanopore data to develop a new workflow to reconstruct 233 MAGs-six novel bacterial orders, 20 families, 66 genera, and 154 species-from Lake Shunet, a secluded meromictic lake in Siberia. With our workflow, the average N50 of reconstructed MAGs greatly increased 10-40-fold compared to when the conventional Illumina assembly and binning method were used. More importantly, six complete MAGs were recovered from our datasets. The recovery of 154 novel species MAGs from a rarely explored lake greatly expands the current bacterial genome encyclopedia. Chen and colleagues develop a workflow for assembling high quality metagenome-associated genomes for microbial species using long and short reads, in this case from a meromictic lake. A full, detailed workflow is provided in for use by the community.

Журнал: COMMUNICATIONS BIOLOGY

Выпуск журнала: Vol. 4, Is. 1

Номера страниц: 996

ISSN журнала: 23993642

Место издания: BERLIN

Издатель: NATURE PORTFOLIO

• Chen Yu-Hsiang (Natl Taiwan Univ, Taiwan Int Grad Program, Bioinformat Program, Taipei, Taiwan; Acad Sinica, Taiwan Int Grad Program, Bioinformat Program, Inst Informat Sci, Taipei, Taiwan; Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan)
• Chiang Pei-Wen (Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan)
• Rogozin Denis Yu (Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia; Siberian Fed Univ, Krasnoyarsk, Russia)
• Degermendzhy Andrey G. (Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia)
• Chiu Hsiu-Hui (Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan)
• Tang Sen-Lin (Acad Sinica, Taiwan Int Grad Program, Bioinformat Program, Inst Informat Sci, Taipei, Taiwan; Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan)

• Web of Science Core Collection
• Scopus
• Ядро РИНЦ (eLIBRARY.RU)