Тип публикации: статья из журнала
Год издания: 2017
Идентификатор DOI: 10.1016/j.nanoso.2017.03.011
Ключевые слова: Bactericidal, DNA damage, Nanoparticles, Phytogenic, Rhizophora mangle
Аннотация: The present study reports phytogenic synthesis of nanoparticles using aqueous extract of Rhizophora mangle. The synthesized nanoparticles were characterized using UV–Visible spectroscopy with maximum absorption at 400 nm. Fourier Transform Infrared spectroscopy (FT-IR) studies revealed the presence of functional groups mediating the synthesis of nanoparticles. X-ray diffraction (XRD) displayed Bragg's intensities at 2? angle which confirmed the crystalline nature of the nanoparticles. Transmission Electron Microscopy (TEM) revealed size and shape of the nanoparticles with polydispersity of nanoparticles and size of 10–60 nm. The synthesized nanoparticles expressed antibacterial activity by well diffusion assay, broth micro dilution assay, minimal inhibitory concentration and CFU assay against selective human pathogens. Nanoparticles were more effective against S. aureus (MTCC 7443) followed by B. subtilis (MTCC 121), E. coli (MTCC 7410) and S. typhi (MTCC 7407). The possible mode of action of nanoparticles was studied by treating silver nanoparticles with pathogenic DNA which showed defamed and damage DNA in comparison with control DNA. © 2017 Elsevier B.V.
Издание
Журнал: Nano-Structures and Nano-Objects
Выпуск журнала: Vol. 10
Номера страниц: 112-115
Персоны
- Syed B. (Department of Biotechnology, JSS Science and Technology University, JSS Technical Institutional Campus, Mysore, India ; Laboratory of Biotechnology of New Materials Siberian Federal University, Siberia, Russian Federation)
- Bisht N.
- S. Bhat P.
- Nikhil Karthik R.
- Prasad A.
- Dhananjaya B.L.
- Satish S.
- Prasad H.
- Nagendra Prasad N.N.
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