Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.3389/fevo.2021.626752

Ключевые слова: barcode index number, cryptic diversity, gracillariidae, lepidoptera, mitonuclear discordance, new candidate species, rad sequencing, wolbachia

Аннотация: Gracillariidae is the most species-rich leaf-mining moth family with over 2,000 described species worldwide. In Europe, there are 263 valid named species recognized, many of which are difficult to identify using morphology only. Here we explore the use of DNA barcodes as a tool for identification and species discovery in European gracillariids. We present a barcode library including 6,791 COI sequences representing 242 of the 263 (92%) resident species. Our results indicate high congruence between morphology and barcodes with 91.3% (221/242) of European species forming monophyletic clades that can be identified accurately using barcodes alone. The remaining 8.7% represent cases of non-monophyly making their identification uncertain using barcodes. Species discrimination based on the Barcode Index Number system (BIN) was successful for 93% of species with 7% of species sharing BINs. We discovered as many as 21 undescribed candidate species, of which six were confirmed from an integrative approach; the other 15 require additional material and study to confirm preliminary evidence. Most of these new candidate species are found in mountainous regions of Mediterranean countries, the South-Eastern Alps and the Balkans, with nine candidate species found only on islands. In addition, 13 species were classified as deep conspecific lineages, comprising a total of 27 BINs with no intraspecific morphological differences found, and no known ecological differentiation. Double-digest restriction-site associated DNA sequencing (ddRAD) analysis showed strong mitonuclear discrepancy in four out of five species studied. This discordance is not explained by Wolbachia-mediated genetic sweeps. Finally, 26 species were classified as “unassessed species splits” containing 71 BINs and some involving geographical isolation or ecological specialization that will require further study to test whether they represent new cryptic species. © Copyright © 2021 Lopez-Vaamonde, Kirichenko, Cama, Doorenweerd, Godfray, Guiguet, Gomboc, Huemer, Landry, Laštůvka, Laštůvka, Lee, Lees, Mutanen, van Nieukerken, Segerer, Triberti, Wieser and Rougerie.

Журнал: Frontiers in Ecology and Evolution

Выпуск журнала: Vol. 9

Номера страниц: 626752

ISSN журнала: 2296701X

Издатель: Frontiers Media S.A.

• Lopez-Vaamonde Carlos (URZF, INRAE, Orleans, France; Univ Tours, IRBI, UMR 7261, CNRS, Tours, France)
• Kirichenko Natalia (RAS, SB, Sukachev Inst Forest, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia; Siberian Fed Univ, Krasnoyarsk, Russia)
• Cama Alain (La Chapelle sur Loire, France)
• Doorenweerd Camiel (Univ Hawaii, Coll Trop Agr & Human Resources, Dept Plant & Environm Protect Sci, Honolulu, HI USA; Nat Biodivers Ctr, Leiden, Netherlands)
• Godfray H.CharlesJ. (Univ Oxford, Dept Zool, Oxford, England)
• Guiguet Antoine (Penn State Univ, Dept Biol, University Pk, PA 16802 USA)
• Gomboc Stanislav (Gancani, Beltinci, Slovenia)
• Huemer Peter (Tiroler Landesmuseen BetriebsgesmbH Sammlungs & F, Hall In Tirol, Austria)
• Landry Jean-Francois (Agr & Agri Food Canada, Ottawa Res & Dev Ctr, Ottawa, ON, Canada)
• Lastuvka Ales (Prostějov, Czech Republic)
• Lastuvka Zdenek (Mendel Univ Brno, Fac Agron, Brno, Czech Republic)
• Lee Kyung Min (Univ Oulu, Ecol & Genet Res Unit, Oulu, Finland)
• Lees David C. (Nat Hist Museum, Dept Life Sci, London, England)
• Mutanen Marko (Univ Oulu, Ecol & Genet Res Unit, Oulu, Finland)
• van Nieukerken Erik J. (Nat Biodivers Ctr, Leiden, Netherlands)
• Segerer Andreas H. (Staatliche Nat Wissensch Sammlungen Bayerns, Zool Staatssammlung Munchen SNSB ZSM, Munich, Germany)
• Triberti Paolo (Museo Civ Storia Nat, Zool, Verona, Italy)
• Wieser Christian (Landesmuseum Karnten, Abt Zool Sammlungs & Wissensch Zentrum, Klagenfurt, Austria)
• Rougerie Rodolphe (Univ Antilles, Inst Systemat Evolut Biodivers ISYEB, Museum Natl Hist Nat, CNRS,Sorbonne Univ,EPHE, Paris, France)

• Scopus
• Web of Science Core Collection
• Ядро РИНЦ (eLIBRARY.RU)