Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1007/s00216-020-02777-4

Ключевые слова: betulin derivatives, stability constants, haarhoff-van der linde function, high-affinity interaction, inclusion complexes, electromigration dispersion

Аннотация: Complexation plays an important role in many biological phenomena, the analysis of different samples, optimization of separation processes. and increasing the pharmacological activity of drugs. This paper discusses the features of using mobility shift affinity capillary electrophoresis for studying strong complexation. Electrophoretic peaks for this case are often triangular. It was shown that the use of electrophoretic mobility obtained from the peak apex time to calculate binding constants leads to significant systematic and random errors, and the parameter a t of the Haarhoff-Van der Linde function should be used instead of the apex time. Distorted triangular peaks with dips were shown to be observed at too high a ratio of analyte concentration in the sample to ligand concentration in the background electrolyte. and the peaks and parameter at significantly shifted. It was found that the permissible excess of analyte concentration over ligand concentration was approximately 10-35, provided that the parameter a t was used, but the peak shape should be used as a landmark, and only triangular peaks without dips should be fitted with the function. The lowest possible analyte concentration should be utilized, which allows the use of a wider range of ligand concentration leading to higher precision of determining the binding constants values. Kinetically labile 1:1 complexes between (2-hydroxypropyl)-gamma-cyclodextrin (HP-gamma-CD) and betulin 3,28-diphthalate (DPhB) and betulin 3,28-disuccinate (DScB) were studied as an example. The binding constant logarithms at 25 degrees C are 7.23 +/- 0.03 and 7.13 +/- 0.10 for the HP-gamma-CD complexes of DPhB and DScB, respectively.

Журнал: ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Выпуск журнала: Vol. 412, Is. 23

Номера страниц: 5615-5625

ISSN журнала: 16182642

Место издания: HEIDELBERG

Издатель: SPRINGER HEIDELBERG

• Sursyakova Viktoria V. (RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Chem & Chem Technol SB, Akademgorodok 50-24, Krasnoyarsk 660036, Russia)
• Levdansky Vladimir A. (RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Chem & Chem Technol SB, Akademgorodok 50-24, Krasnoyarsk 660036, Russia)
• Rubaylo Anatoly I. (RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Chem & Chem Technol SB, Akademgorodok 50-24, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Svobodny Pr 79, Krasnoyarsk 660041, Russia; RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Akademgorodok 50, Krasnoyarsk 660036, Russia)

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