Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2023

Идентификатор DOI: 10.3390/s23052865

Аннотация: <jats:p>Saliva is one of the most significant biological liquids for the development of a simple, rapid, and non-invasive biosensor for training load diagnostics. There is an opinion that enzymatic bioassays are more relevant in terms of biology. The present paper is aimed at investigating the effects of saliva samples, upon altering the lactate content, on the activity of a multi-enzyme, namely lactate dehydrogenase + NAD(P)H:FMN-oxidoreductase + luciferase (LDH + Red + Luc). Optimal enzymes and their substrate composition of the proposed multi-enzyme system were chosen. During the tests of the lactate dependence, the enzymatic bioassay showed good linearity to lactate in the range from 0.05 mM to 0.25 mM. The activity of the LDH + Red + Luc enzyme system was tested in the presence of 20 saliva samples taken from students whose lactate levels were compared by the Barker and Summerson colorimetric method. The results showed a good correlation. The proposed LDH + Red + Luc enzyme system could be a useful, competitive, and non-invasive tool for correct and rapid monitoring of lactate in saliva. This enzyme-based bioassay is easy to use, rapid, and has the potential to deliver point-of-care diagnostics in a cost-effective manner.</jats:p>

Журнал: Sensors

Выпуск журнала: Т.23, №5

Номера страниц: 2865

ISSN журнала: 14248220

Издатель: Molecular Diversity Preservation International

• Zhukova Galina V. (Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia)
• Sutormin Oleg S. (Department of Chemistry, Institute of Natural and Technical Sciences, Surgut State University, 628412 Surgut, Russia)
• Sukovataya Irina E. (Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia)
• Maznyak Natalya V. (School of Non-Ferrous Metals and Materials Science, Siberian Federal University, 660041 Krasnoyarsk, Russia)
• Kratasyuk Valentina A. (Photobiology Laboratory, Institute of Biophysics, Federal Research Center ‘Krasnoyarsk Science Center’, Siberian Branch of the Russian Academy of Sciences, 660036 Krasnoyarsk, Russia)

• Ядро РИНЦ (eLIBRARY.RU)