Photo-immobilization of proteins on carbons | Научно-инновационный портал СФУ

Photo-immobilization of proteins on carbons

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1016/j.jphotobiol.2019.111675

Ключевые слова: Bovine serum albumin, Carbons, Graphene oxide, Graphene oxide modified with SO2, Graphite, Photo-immobilization, α-amylaseamylase, 9000-90-2, 9000-92-4, 9001-19-8, carbon, 7440-44-0, graphite, 7782-42-5, protein, 67254-75-5

Аннотация: The photofunctionalization of three different carbons with two proteins was studied at room temperature. Water solutions of bovine serum albumin, BSA, and α-amylase, AA, were photolyzed at 21 °C in the presence of graphite microparticles (6.20 μm), MPG, graphene oxide, MPGO, and graphene oxide modified with SO2, mMPGO. The insertion of BSA on carbon matrixes occurred with a deoxygenation reaction, most likely due to a dehydration step of a water molecule. XPS, TOC and TGA, showed that the BSA photo-insertion on MPG was highly efficient with 34.9% of the weight of MPG after photolysis, with an initial concentration of 1 g∙L−1 of BSA. A high yield of AA photoinsertion on the carbons was also obtained. The calculated weight of AA inserted on MPG and MPGO after photolysis was 22.30% and 18.08%, respectively, with respect to the initial weight of carbon, when the initial concentration of AA was 60 mg∙L−1. AA immobilized on MPG was active while the enzyme on MPGO showed a smaller activity, within the experimental error. Although a certain extent of denaturalization of both proteins was observed during photolysis, the molecular weight and composition changed very little during the photolysis, which would produce mainly conformational changes and isomerization reactions. © 2019 Elsevier B.V.

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Издание

Журнал: Journal of Photochemistry and Photobiology B: Biology

Выпуск журнала: Vol. 202

Номера страниц: 111675

ISSN журнала: 10111344

Издатель: Elsevier B.V.

Авторы

  • Humeres Eduardo (Univ Fed Santa Catarina, Dept Quim, Florianopolis, SC, Brazil)
  • Canle Moises (Univ A Coruna, Grp Reactividade Quim & Fotorreactividade React, Dept Quim, Fac Ciencias, E-15071 La Coruna, Spain; Univ A Coruna, CICA, E-15071 La Coruna, Spain)
  • Lopes Cristiane Nunes (Univ Fed Santa Catarina, Dept Engn Quim & Alimentos, Florianopolis, SC, Brazil)
  • Santaballa J.Arturo (Univ A Coruna, Grp Reactividade Quim & Fotorreactividade React, Dept Quim, Fac Ciencias, E-15071 La Coruna, Spain; Univ A Coruna, CICA, E-15071 La Coruna, Spain)
  • Debacher Nito Angelo (Univ Fed Santa Catarina, Dept Quim, Florianopolis, SC, Brazil)
  • Moreira Regina de F. P. M. (Univ Fed Santa Catarina, Dept Engn Quim & Alimentos, Florianopolis, SC, Brazil)
  • Safin Vladimir (Univ A Coruna, Grp Reactividade Quim & Fotorreactividade React, Dept Quim, Fac Ciencias, E-15071 La Coruna, Spain; Univ A Coruna, CICA, E-15071 La Coruna, Spain; Siberian Fed Univ, Dept Chem & Technol Nat Energy Carriers & Carbon, Krasnoyarsk, Russia)
  • Fernandez Perez M.Isabel (Univ A Coruna, Grp Reactividade Quim & Fotorreactividade React, Dept Quim, Fac Ciencias, E-15071 La Coruna, Spain; Univ A Coruna, CICA, E-15071 La Coruna, Spain)

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