Evaluation of the effect of background electrolytecomposition and independence of parameters indetermining binding constants of betulin derivatives to β-and dimethyl-β-cyclodextrins by affinity capillaryelectrophoresis : научное издание

Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.1002/jssc.202200453

Ключевые слова: drug delivery, host-guest complex, inclusion complex, pentacyclic lupane triterpenoids, solubilization

Аннотация: The values of the apparent binding constants for β-cyclodextrin complexes ofbetulin derivatives determined by mobility shift affinity capillary electrophoresiswere found to be independent of the composition of the two background elec-trolytes used (tetraborate buffer, pH 9.18, and phosphate buffer, pH 8.00, both ofthem with 20 mM ionic strength). It has been found that if there is not a con-stant plateau on the binding curve then four independent parameters can bedetermined: binding constants (also referred to as stability, association, or for-mation constants) and ionic mobilities of 1:1 and 1:2 complexes. However, at least10–12 data points in the binding curve should be used to reliably estimate theparameters. For the first time, the apparent binding constants for complexes ofester betulin derivatives with dimethyl-β-cyclodextrin have been determined bymobility shift affinity capillary electrophoresis. The logarithms of the constantsfor1:1and1:2complexesat25◦C for betulin 3,28-diphthalate with a 95% confi-dence interval are 4.98 (4.95–5.01) and 7.52 (7.26–7.68); for betulin 3,28-disulfate,the values are 4.97 (4.89–5.03) and 8.24 (6.82–8.52). It has been found that betulin3,28-disuccinate forms only a 1:1 complex and the binding constant logarithm is5.25±0.02.

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Издание

Журнал: Journal of Separation Science

Выпуск журнала: Т. 45, № 19

Номера страниц: 3745-3753

ISSN журнала: 16159306

Издатель: Wiley Interscience

Персоны

Sursyakova Viktoria V. (Institute of Chemistry and ChemicalTechnology SB RAS, Federal ResearchCenter “Krasnoyarsk Science Center SB RAS”)
Levdansky Vladimir A. (Institute of Chemistry and ChemicalTechnology SB RAS, Federal ResearchCenter “Krasnoyarsk Science Center SB RAS”)
Rubaylo Anatoly I. (School of Non-Ferrous Metals andMaterial Science, Siberian FederalUniversity)

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