Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.3390/nano12020249

Ключевые слова: biogenic ferrihydrite nanoparticles, energy transfer, molecular docking, protein stability, the binding mechanism

Аннотация: The synthesis of nanoparticles inside microorganisms is an economical alternative to chemical and physical methods of nanoparticle synthesis. In this study, ferrihydrite nanoparticles synthesized by Klebsiella oxytoca bacterium in special conditions were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDS), small-angle X-ray (SAXS), UV-Vis spectroscopy, fluorescence, fluorescence resonance energy transfer (FRET), and molecular docking. The morphology and the structure of the particles were characterized by means of SEM and SAXS. The elemental content was determined by means of the EDS method. The absorption properties of the ferrihydrite nanoparticles were investigated by UV-Vis spectroscopy. The binding mechanism of the biogenic ferrihydrite nanoparticles to Bovine Serum Albumin (BSA) protein, studied by fluorescence, showed a static and weak process, combined with FRET. Protein denaturation by temperature and urea in the presence of the ferrihydrite nanoparticles demonstrated their influence on the unfolding process. The AutoDock Vina and UCSF Chimera programs were used to predict the optimal binding site of the ferrihydrite to BSA and to find the location of the hydrophobic cavities in the sub-domain IIA of the BSA structure. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Журнал: Nanomaterials

Выпуск журнала: Vol. 12, Is. 2

Номера страниц: 249

ISSN журнала: 20794991

Издатель: MDPI

• Cazacu N. (Department of Electricity, Solid State and Biophysics, Faculty of Physics, University of Bucharest, Măgurele, RO-077125, Romania)
• Chilom C.G. (Department of Electricity, Solid State and Biophysics, Faculty of Physics, University of Bucharest, Măgurele, RO-077125, Romania)
• Iftimie S. (Department of Electricity, Solid State and Biophysics, Faculty of Physics, University of Bucharest, Măgurele, RO-077125, Romania)
• Bălășoiu M. (Department of Nuclear Physics, “Horia Hulubei” National Institute of Physics and Nuclear Engineering, Măgurele, RO-077125, Romania, Joint Institute for Nuclear Research, Dubna, 141980, Russian Federation, Moscow Institute of Physics and Technology, Dolgoprudniy, 141701, Russian Federation)
• Ladygina V.P. (Federal Research Center KSC, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation)
• Stolyar S.V. (Federal Research Center KSC, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation, Physics Department, School of Engineering Physics and Radio Electronics, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation, Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation)
• Orelovich O.L. (Joint Institute for Nuclear Research, Dubna, 141980, Russian Federation)
• Kovalev Y.S. (Joint Institute for Nuclear Research, Dubna, 141980, Russian Federation)
• Rogachev A.V. (Joint Institute for Nuclear Research, Dubna, 141980, Russian Federation, Moscow Institute of Physics and Technology, Dolgoprudniy, 141701, Russian Federation)

• Scopus
• Web of Science Core Collection
• Ядро РИНЦ (eLIBRARY.RU)