Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2016

Идентификатор DOI: 10.1002/jctb.4592

Ключевые слова: wild-type strain Cupriavidus eutrophus, B10646, growth kinetics, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), physicochemical and mechanical properties, Wild-type strain Cupriavidus eutrophus B10646, Biomaterials, Biomechanics, Chemical industry, Cultivation, Mechanical properties, Organic compounds, Polymers, Sodium, 3-Hydroxyhexanoate, Bacterial strains, Cultivation conditions, Kinetic properties, Physico-chemical and mechanical properties, Physiological range, Wild-type strain, Strain, copolymer, glucose, hexanoic acid, nitrogen, Article, bacterial strain, biomass, Cupriavidus, Cupriavidus eutrophus, differential scanning calorimetry, enzyme substrate, kinetics, measurement accuracy, nonhuman, synthesis, thermal analysis, wild type

Аннотация: BACKGROUNDP(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). The most common approach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this study was to optimize the process of production of P(3HB-co-3HHx) copolymers containing high molar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophusB10646. RESULTSKinetic properties of C. eutrophusB10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophusB10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSIONFor the first time, cultivation conditions of Cupriavidus eutrophusB10646 enabled production of high biomass yields (5-6gL(-1)) and high content of the polymer (60-75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers. (c) 2014 Society of Chemical Industry

Журнал: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Выпуск журнала: Vol. 91, Is. 2

Номера страниц: 416-425

ISSN журнала: 02682575

Место издания: HOBOKEN

Издатель: WILEY-BLACKWELL

• Volova T.G. (Siberian Federal University)
• Zhila N.O. (Siberian Federal University)
• Sukovatiy A.G. (Institute of Biophysics SB RAS)
• Syrvacheva D.A. (Siberian Federal University)

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