Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction | Научно-инновационный портал СФУ

Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1134/S1607672920020106

Ключевые слова: bacterial luciferase, bioluminescence, diffusional restriction, enzyme reaction intermediate, molecular dynamics method, stopped-flow technique, viscous microenvironment

Аннотация: Abstract: The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1–1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in the decay rate following a power function with an exponent of 0.82–0.84. Using molecular dynamics methods, it was shown that the presence of glycerol and sucrose molecules causes a change in the mobility of the amino acid residues in the active center of luciferase, particularly those responsible for binding of flavin. The results obtained are indicative of two opposite effects of viscous media with glycerol and sucrose: (1) destabilization of 4a-hydroperoxyflavin due to a change in the structural and dynamic properties of the protein and (2) stabilization of this intermediate by the decrease in the diffusion rate of its decay products. © 2020, Pleiades Publishing, Ltd.

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Издание

Журнал: Doklady Biochemistry and Biophysics

Выпуск журнала: Vol. 492, Is. 1

Номера страниц: 162-165

ISSN журнала: 16076729

Издатель: Pleiades Publishing

Авторы

  • Lisitsa A.E. (Siberian Fed Univ, Krasnoyarsk, Russia)
  • Sukovatyi L.A. (Siberian Fed Univ, Krasnoyarsk, Russia)
  • Kratasyuk V.A. (Siberian Fed Univ, Krasnoyarsk, Russia; Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia)
  • Nemtseva E.V. (Siberian Fed Univ, Krasnoyarsk, Russia; Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia)

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