Тип публикации: статья из журнала
Год издания: 2020
Идентификатор DOI: 10.1099/ijsem.0.004453
Ключевые слова: archaea, halotolerant, lake tus, methanogen, methanolobus, methylotrophic, psychrotolerant, saline lake, siberia
Аннотация: A halotolerant, psychrotolerant and methylotrophic methanogen, strain SY-01T, was isolated from the saline Lake Tus in Siberia. Cells of strain SY-01T were non-motile, cocci and 0.8-1.0 µm in diameter. The only methanogenic substrate utilized by strain SY-01T was methanol. The temperature range of growth for strain SY-01T was from 4 to 40 °C and the optimal temperature for growth was 30 °C. The pH range of growth was from pH 7.2 to 9.0, with optimal growth at pH 8.0. The NaCl range of growth was 0-1.55 M with optimal growth at 0.51 M NaCl. The G+C content of the genome of strain SY-01T was 43.6 mol % as determined by genome sequencing. Phylogenetic analysis revealed that strain SY-01T was most closely related to Methanolobus zinderi SD1T (97.3 % 16S rRNA gene sequence similarity), and had 95.5-97.2 % similarities to other Methanolobus species with valid names. Genome relatedness between strain SY-01T and DSM 21339T was computed using average nucleotide identity and digital DNA- DNAhybridization, which yielded values of 79.7 and 21.7 %, respectively. Based on morphological, phenotypic, phylogenetic and genomic relatedness data presented here, it is evident that strain SY-01T represents a novel species of the genus Methanolobus, and the name Methanolobus halotolerans sp. nov. is proposed. The type strain is SY-01T (=BCRC AR10051T=NBRC 113166 T=DSM 107642T). © 2020 The Authors.
Издание
Журнал: International Journal of Systematic and Evolutionary Microbiology
Выпуск журнала: Vol. 70, Is. 10
Номера страниц: 5586-5593
Персоны
- Shen Ya (Natl Chung Hsing Univ, Dept Life Sci, Taichung, Taiwan)
- Chen Sheng-Chung (Natl Chung Hsing Univ, Dept Life Sci, Taichung, Taiwan; Sanming Univ, Sch Resources & Chem Engn, Sanming 365004, Fujian, Peoples R China; Sanming Univ, Fujian Prov Key Lab Resources & Environm Monitori, Sanming 365004, Fujian, Peoples R China)
- Lai Mei-Chin (Natl Chung Hsing Univ, Dept Life Sci, Taichung, Taiwan; Natl Chung Hsing Univ, Agr Biotechnol Ctr, Taichung, Taiwan)
- Huang Hsing-Hua (Natl Chung Hsing Univ, Dept Life Sci, Taichung, Taiwan)
- Chiu Hsiu-Hui (Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan)
- Tang Sen-Lin (Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan)
- Rogozin Denis Yu (Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Krasnoyarsk 660041, Russia)
- Degermendzhy Andrey G. (Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Krasnoyarsk 660041, Russia)
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