Тип публикации: статья из журнала
Год издания: 2007
Идентификатор DOI: 10.1002/bio.952
Ключевые слова: obelin; bioluminescence immunoassay; infective agents
Аннотация: The Ca2+-regulated photoprotein obelin has been examined as a label for bioluminescence immunoassay of infective agents. The hepatitis B virus (HbsAg) and the bacteria Escherichia coli and Shigella sonnei lipopolysaccharide (LPS) were chosen as model antigens. Chemically synthesized obelin-corresponding antibody conjugates were used in a solid-phase microplate immunoassay. The sensitivities achieved by the assay were 0.25 ng/mL for S. sonnei LPS and 0.375 ng/mL for HbsAg. A novel, filter-based immunoassay to determine bacterial admixtures in the environment was proposed. The NanoCeram filters were effectively applied to 'trap' and pre-concentrate pathogens from samples under study for the purposes of further detection and measurement of the absorbed material by bioluminescence immunoassay. Copyright (C) 2007 John Wiley & Sons, Ltd.
Издание
Журнал: LUMINESCENCE
Выпуск журнала: Vol. 22, Is. 3
Номера страниц: 215-220
ISSN журнала: 15227235
Место издания: CHICHESTER
Издатель: JOHN WILEY & SONS LTD
Персоны
- Frank L. (Institute of Biophysics,Russian Academy of Sciences Siberian Branch)
- Markova S. (Institute of Biophysics,Russian Academy of Sciences Siberian Branch)
- Vysotski E. (Institute of Biophysics,Russian Academy of Sciences Siberian Branch)
- Gitelson I. (Institute of Biophysics,Russian Academy of Sciences Siberian Branch)
- Remmel N. (Krasnoyarsk State University)
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