Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2011

Идентификатор DOI: 10.1134/S1995425511010024

Ключевые слова: free-living uncultivated bacterioplankton, amino acids, PCR-DGGE, microecosystems, 16S ribosomal RNA, amino acid, bacterioplankton, biological uptake, community structure, cultivation, dominance, electrokinesis, eutrophic environment, gel, genetic analysis, molecular analysis, polymerase chain reaction, substrate, Bacteria (microorganisms)

Аннотация: Water bacterioplankton species (clones) feeding on lysine and glycine were revealed in experimental microecosystems using nonsterile cultivation. The structure of the community was analyzed by separation of amplified fragments of the bacterial 16S rRNA gene of by denaturing gradient gel electrophoresis. The dominant species (clones) of the bacterioplankton were identified by molecular cloning and determining the nucleotide sequence of part of the 16S gene of ribosomal RNA. It was found that glycine, one of the prevalent amino acids of a eutrophic reservoir, and lysine, whose content in natural water is lower, are nutrient substrates for various subdominant bacterioplankton species (clones). Thus, free-living bacterial species are highly specialized for the uptake of individual amino acids.

Журнал: CONTEMPORARY PROBLEMS OF ECOLOGY

Выпуск журнала: Vol. 4, Is. 1

Номера страниц: 8-14

ISSN журнала: 19954255

Место издания: NEW YORK

Издатель: SPRINGER

• Kolmakova O.V. (Siberian Federal University)
• Trusova M.Y. (Institute of Biophysics)

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