Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect : научное издание | Научно-инновационный портал СФУ

Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect : научное издание

Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.3390/biomedicines9030323

Ключевые слова: ferrihydrite nanoparticles, concentration effect, microorganisms klebsiella oxytoca, neutrophilic granulocytes, chemiluminescence, toxicology

Аннотация: Biogenic ferrihydrite nanoparticles were synthesized as a result of the cultivation of Klebsiella oxytoca microorganisms. The distribution of nanoparticles in the body of laboratory animals and the physical properties of the nanoparticles were studied. Th Biogenic ferrihydrite nanoparticles were synthesized as a result of the cultivation of Klebsiella oxytoca microorganisms. The distribution of nanoparticles in the body of laboratory animals and the physical properties of the nanoparticles were studied. The synthesized ferrihydrite nanoparticles are superparamagnetic at room temperature, and the characteristic blocking temperature is 23–25 K. The uncompensated moment of ferrihydrite particles was determined to be approximately 200 Bohr magnetons. In vitro testing of different concentrations of ferrihydrite na-noparticles for the functional activity of neutrophilic granulocytes by the chemiluminescence method showed an increase in the release of primary oxygen radicals by blood phagocytes when exposed to a minimum concentration and a decrease in secondary radicals when exposed to a maximum concentration. In vivo testing of ferrihydrite nanoparticles on Wister rats showed that a suspension of ferrihydrite nanoparticles has chronic toxicity, since it causes morphological changes in organs, mainly in the spleen, which are characterized by the accumulation of hemosiderin na-noparticles (stained blue according to Perls). Ferrihydrite can also directly or indirectly stimulate the proliferation and intracellular regeneration of hepatocytes. The partial detection of Perls-positive cells in the liver and kidneys can be explained by the rapid elimination from organs and the high dispersion of the nanomaterial. Thus, it is necessary to carry out studies of these processes at the systemic level, since the introduction of nanoparticles into the body is characterized by adaptive-proliferative processes, accompanied by the development of cell dystrophy and tension of the phagocytic system. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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Издание

Журнал: BIOMEDICINES

Выпуск журнала: Vol. 9, Is. 3

Номера страниц: 323

ISSN журнала: 22279059

Место издания: BASEL

Издатель: MDPI

Авторы

  • Stolyar Sergey V. (RAS, Kirensky Inst Phys, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia; RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Dept Biophys, Krasnoyarsk 660041, Russia)
  • Kolenchukova Oksana A. (Siberian Fed Univ, Dept Biophys, Krasnoyarsk 660041, Russia; RAS, Sci Res Inst Med Problems North, Fed Res Ctr KSC SB, Krasnoyarsk 660022, Russia)
  • Boldyreva Anna V. (RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia)
  • Kudryasheva Nadezda S. (Siberian Fed Univ, Dept Biophys, Krasnoyarsk 660041, Russia; RAS, Inst Biophys, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia)
  • Gerasimova Yulia V. (RAS, Kirensky Inst Phys, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Dept Biophys, Krasnoyarsk 660041, Russia)
  • Krasikov Alexandr A. (RAS, Kirensky Inst Phys, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia)
  • Yaroslavtsev Roman N. (RAS, Kirensky Inst Phys, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia; RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia)
  • Bayukov Oleg A. (RAS, Kirensky Inst Phys, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia)
  • Ladygina Valentina P. (RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr KSC SB, Krasnoyarsk 660036, Russia)
  • Birukova Elena A. (RAS, Sci Res Inst Med Problems North, Fed Res Ctr KSC SB, Krasnoyarsk 660022, Russia)

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