Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2007

Идентификатор DOI: 10.1016/j.enzmictec.2006.05.003

Ключевые слова: bioluminescence; bacterial luciferase; NADH : FMN-oxidoreductase; immobilization; bioluminescent assays

Аннотация: A disk-shaped reagent based on bacterial NADH:FMN-oxidoreductase and luciferase immobilized into a starch gel is developed. The reagent characteristics, such as activity and stability depend on the time of drying, the initial luciferase preparation content, the source of the starch and its gel concentration. The experimental error of bioluminescence determination does not exceed 10-15%. The method of immobilization into gel provides the coimmobilization luciferase and NADH:FMN-oxidoreductase and their substrates. On the basis of these reagents, the multifunctional immobilized biosensor for bioluminescent assay in medicine, ecology and other fields may be developed. (c) 2006 Elsevier Inc. All rights reserved.

Журнал: ENZYME AND MICROBIAL TECHNOLOGY

Выпуск журнала: Vol. 40, Is. 2

Номера страниц: 343-346

ISSN журнала: 01410229

Место издания: NEW YORK

Издатель: ELSEVIER SCIENCE INC

• Esimbekova E.N. (Institute of Biophysics,Russian Academy of Sciences, SB)
• Kratasyuk V.A. (Krasnoyarsk State University)
• Torgashina I.G. (Krasnoyarsk State University)

• Web of Science Core Collection
• Scopus
• Ядро РИНЦ (eLIBRARY.RU)