Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch (Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing

Тип публикации: статья из журнала

Год издания: 2014

Идентификатор DOI: 10.1007/s11627-014-9619-z

Ключевые слова: Cytogenetics, Genomic instability, Haploid tissue culture, Megagametophyte, Microsatellite loci, Siberian larch, Larix sibirica Ledeb, Somaclonal variation, Larix, Larix sibirica

Аннотация: The objective of this study was to obtain a genetically stable haploid in vitro-derived line from Siberian larch (Larix sibirica Ledeb.) using megagametophyte explants, which then could be used for different molecular genetic studies, including whole genome de novo sequencing. However, cytogenetic analysis and genotyping of 11 microsatellite loci showed high levels of genomic instability and a high frequency of mutation in the obtained megagametophyte-derived callus cultures. All cultures contained new mutations in one or more microsatellite loci.

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Издание

Журнал: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Выпуск журнала: Vol. 50, Is. 5

Номера страниц: 655-664

ISSN журнала: 10545476

Место издания: NEW YORK

Издатель: SPRINGER

Персоны

Krutovsky Konstantin V. (Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgen Inst, D-37077 Gottingen, Germany; Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA; Russian Acad Sci, NI Vavilov Inst Gen Genet, Moscow 119333, Russia; Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia)
Tretyakova Iraida N. (Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia; Russian Acad Sci, Lab Forest Genet & Select, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia)
Oreshkova Nataliay V. (Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia; Russian Acad Sci, Lab Forest Genet & Select, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia)
Pak Maria E. (Russian Acad Sci, Lab Forest Genet & Select, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia)
Kvitko Olga V. (Russian Acad Sci, Lab Forest Genet & Select, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia)
Vaganov Eugene A. (Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Dept Ecol & Environm Studies, Inst Econ Management & Environm Studies, Krasnoyarsk 660041, Russia)

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