Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha

Тип публикации: статья из журнала

Год издания: 2013

Идентификатор DOI: 10.1016/j.resmic.2012.10.008

Ключевые слова: Poly(3-hydroxybutyrate), Granules, PHA cycle, Ralstonia eutropha, Granules, PHA cycle, Poly(3-hydroxybutyrate), Ralstonia eutropha, poly(3 hydroxybutyric acid), article, bacterial growth, bacterial strain, bacterium culture, cell division, cell granule, cell size, controlled study, Cupriavidus necator, electron microscopy, nonhuman, polymer production, priority journal, Cupriavidus necator, Cytoplasmic Granules, Hydroxybutyrates, Microscopy, Electron, Transmission, Polyesters, Bacteria (microorganisms), Cupriavidus necator

Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. (C) 2012 Published by Elsevier Masson SAS on behalf of Institut Pasteur.

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Издание

Журнал: RESEARCH IN MICROBIOLOGY

Выпуск журнала: Vol. 164, Is. 2

Номера страниц: 164-171

ISSN журнала: 09232508

Место издания: AMSTERDAM

Издатель: ELSEVIER SCIENCE BV

Авторы

  • Volova Tatiana G. (Russian Acad Sci, Siberian Branch, Inst Biophys, Lab Chemoautotroph Biosynth, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biotechnol, Krasnoyarsk 660041, Russia)
  • Zhila Natalia O. (Russian Acad Sci, Siberian Branch, Inst Biophys, Lab Chemoautotroph Biosynth, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biotechnol, Krasnoyarsk 660041, Russia)
  • Kalacheva Galina S. (Russian Acad Sci, Siberian Branch, Inst Biophys, Lab Chemoautotroph Biosynth, Krasnoyarsk 660036, Russia)
  • Brigham Christopher J. (MIT, Dept Biol, Cambridge, MA 02139 USA)
  • Sinskey Anthony J. (MIT, Dept Biol, Cambridge, MA 02139 USA; MIT, Hlth Sci Technol Div, Cambridge, MA 02139 USA; MIT, Engn Syst Div, Cambridge, MA 02139 USA)

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