Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2015

Идентификатор DOI: 10.2174/1386207318666150917100011

Ключевые слова: Luciferase, luciferin, photoprotein, bioluminescence, mutagenesis, luciferase-based assay, bioimaging, reporter assay

Аннотация: Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescence-based analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc.

Журнал: COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Выпуск журнала: Vol. 18, Is. 10

Номера страниц: 919-929

ISSN журнала: 13862073

Место издания: SHARJAH

Издатель: BENTHAM SCIENCE PUBL LTD

• Frank L.A. (Siberian Federal University)

• Web of Science Core Collection
• Scopus
• Ядро РИНЦ (eLIBRARY.RU)