Тип публикации: статья из журнала
Год издания: 2019
Идентификатор DOI: 10.1016/j.mimet.2019.02.009
Ключевые слова: Bacterial cellulose, Bio-functionalization, Bupleurum aureum, Multi-drug resistance pathogens, Silver nanobactericides, Zinc oxide nanobactericides
Аннотация: The present study describes the synthesis of silver and zinc oxide nanobactericides from the phytogenic source Bupleurum aureum. The synthesized nanobactericides were characterized and evaluated for bio-functionalization onto bacterial cellulose membrane which was synthesized by Komagataeibacterxylinus B-12068 culture strain. The synthesis of nanobacterides were initially confirmed using UV–Visible spectroscopy which indicated localized surface resonance (LSPR) peaks at 415 nm for silver nanobactericides and 280 nm for zinc nanobactericides. The nature of the capping agent for synthesized nanobactericides was predicted using FTIR which confirmed the presence of functional moieties. XRD analysis revealed their crystalline nature while morphological characteristics were studied using TEM which confirmed the polydispersity of nanobactericides with the average size in the range of 20–25 nm. The nanobactericides were tested for their antimicrobial activity against seven multi-drug resistant pathogens which were clinically isolated from patients suffering from a myriad of microbial infections. The tested pathogens had antimicrobial resistance to ten different antibiotics and have been reported to be the major cause of nosocomial infections. The nanobactericides displayed significant activity against the test pathogens. Silver nanobactericides showed the highest activity against Escherichia coli strain 55 with a 24 mm zone of inhibition while zinc oxide nanobactericides displayed the highest activity against methicillin-resistant Staphylococcus aureus (MRSA) with a 20 mm inhibition zone. The bio- functionalized cellulose films (BCF) were characterized using SEM along with physicochemical analysis. The BCF's were evaluated for antibacterial activity against test pathogens which resulted in marked antimicrobial potential against multi-drug resistant bacteria and therefore has the potential to be utilized as an efficient alternative to counter drug resistant pathogens. © 2019 Elsevier B.V.
Журнал: Journal of Microbiological Methods
Выпуск журнала: Vol. 159
Номера страниц: 42-50
ISSN журнала: 01677012
Издатель: Elsevier B.V.
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