Bioluminescent Enzymatic Assay as a Tool for Studying Antioxidant Activity and Toxicity of Bioactive Compounds

Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1111/php.12639

Аннотация: A bioluminescent assay based on a system of coupled enzymatic reactions catalyzed by bacterial luciferase and NADH:FMN-oxidoreductase was developed to monitor toxicity and antioxidant activity of bioactive compounds. The assay enables studying toxic effects at the level of biomolecules and physicochemical processes, as well as determining the toxicity of general and oxidative types. Toxic and detoxifying effects of bioactive compounds were studied. Fullerenols, perspective pharmaceutical agents, nanosized particles, water-soluble polyhydroxylated fullerene-60 derivatives were chosen as bioactive compounds. Two homologous fullerenols with different number and type of substituents, C60O2–4(OH)20–24 and Fe0.5C60(OH) xOy (x + y = 40–42), were used. They suppressed bioluminescent intensity at concentrations >0.01 g L?1 and >0.001 g L?1 for C60O2–4(OH)20-24 and Fe0.5C60(OH)xOy, respectively; hence, a lower toxicity of C60O2–4(OH)20–24 was demonstrated. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic oxidizers; changes in toxicities of general and oxidative type were determined; detoxification coefficients were calculated. Fullerenol C60O2–4(OH)20–24 revealed higher antioxidant ability at concentrations 10?17?10?5 g L?1. The difference in the toxicity and antioxidant activity of fullerenols was explained through their electron donor/acceptor properties and different catalytic activity. Principles of bioluminescent enzyme assay application for evaluating the toxic effect and antioxidant activity of bioactive compounds were summarized and the procedure steps were described. © 2016 The American Society of Photobiology

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Издание

Журнал: Photochemistry and Photobiology

Выпуск журнала: Vol. 93, Is. 2

Номера страниц: 536-540

ISSN журнала: 00318655

Авторы

  • Kudryasheva N.S. (Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation, Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Kovel E.S. (Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation, Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Sachkova A.S. (National Research Tomsk Polytechnic University, Tomsk, Russian Federation)
  • Vorobeva A.A. (National Research Tomsk Polytechnic University, Tomsk, Russian Federation)
  • Isakova V.G. (Institute of Physics SB RAS, Krasnoyarsk, Russian Federation)
  • Churilov G.N. (Institute of Physics SB RAS, Krasnoyarsk, Russian Federation)

Вхождение в базы данных

  • Scopus (цитирований 7)
  • РИНЦ (eLIBRARY.RU) (цитирований 7)
  • Ядро РИНЦ (eLIBRARY.RU)
  • Web of Science Core Collection

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