Redox compounds influence on the NAD(P)H : FMN-oxidoreductase-luciferase bioluminescent system

Тип публикации: статья из журнала

Год издания: 2007

Идентификатор DOI: 10.1039/b608152e

Аннотация: A review of the mechanisms of the exogenous redox compounds in fluence on the bacterial coupled enzyme system: NAD(P)H:FMN-oxidoreductase-luciferase has been done. A series of quinones has been used as model organic oxidants. The three mechanisms of the quinones' effects on bioluminescence were suggested: (1) inhibition of the NADH-dependent redox reactions; (2) interactions between the compounds and the enzymes of the coupled enzyme system; and (3) intermolecular energy migration. The correlation between the kinetic parameters of bioluminescence and the standard redox potential of the quinones proved that the inhibition of redox reactions was the key mechanism by which the quinones decrease the light emission intensity. The changes in the fluorescence anisotropy decay of the endogenous flavin of the enzyme preparations showed the direct interaction between quinones and enzymes. It has been demonstrated that the intermolecular energy migration mechanism played a minor role in the effect of quinones on the bioluminescence. A comparative analysis of the effect of quinones, phenols and inorganic redox compounds on bioluminescent coupled enzyme systems has been carried out.

Ссылки на полный текст

Издание

Журнал: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES

Выпуск журнала: Vol. 6, Is. 1

Номера страниц: 35-40

ISSN журнала: 1474905X

Место издания: CAMBRIDGE

Издатель: ROYAL SOC CHEMISTRY

Авторы

Вхождение в базы данных

Информация о публикациях загружается с сайта службы поддержки публикационной активности СФУ. Сообщите, если заметили неточности.

Вы можете отметить интересные фрагменты текста, которые будут доступны по уникальной ссылке в адресной строке браузера.