Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1016/j.scitotenv.2018.04.405

Ключевые слова: 2,4-DAPG, Bactericidal activity, Bioconjugation, Nano-complex, Silver nanoparticles

Аннотация: The present study emphasizes the need for novel antimicrobial agents to combat the global drug resistant crisis. The development of novel nanomaterials is reported to be of the alternative tool to combat drug resistant pathogens. In present investigation, bioconjugated nano-complex was developed from secondary metabolite secreted from endosymbiont. The endosymbiont capable of secreting antimicrobial metabolite was subjected to fermentation and the culture supernatant was assessed for purification of antimicrobial metabolite via bio-assay guided fraction techniques such as thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and column chromatography. The metabolite was characterized as 2,4-Diacetylphloroglucinol (2,4 DAPG) which was used to develop bioconjugated nano-complex by treating with 1 mM silver nitrate under optimized conditions. The purified metabolite 2,4 DAPG reduced silver nitrate to form bioconjugated nano-complex to form association with silver nanoparticles. The oxidized form of DAPG consists of four hard ligands that can conjugate on to the surface of silver nanoparticles cluster. The bioconjugation was confirmed with UV–visible spectroscopy which displayed the shift and shoulder peak in the absorbance spectra. This biomolecular interaction was further determined by the Fourier-transform spectroscopy (FTIR) and nuclear magnetic resonance (NMR) analyses which displayed different signals ascertaining the molecular binding of 2,4,DAPG with silver nanoparticles. The transmission electron microscopy (TEM) analysis revealed the cluster formation due to bioconjugation. The XRD analysis revealed the crystalline nature of nano-complex with the characteristic peaks indexed to Bragg's reflection occurring at 2θ angle which indicated the (111), (200), (220) and (311) planes. The activity of bioconjugated nano-complex was tested against 12 significant human and phytopathogens. Among all the test pathogens, Shigella flexneri (MTCC 1457) was the most sensitive organisms with 38.33 ± 0.33 zone of inhibition. The results obtained in the present investigation attribute development of nano-complex as one of the effective tools against multi-drug resistant infections across the globe. © 2018 Elsevier B.V.

Журнал: Science of the Total Environment

Выпуск журнала: Vol. 637-638

Номера страниц: 274-281

ISSN журнала: 00489697

Издатель: Elsevier B.V.

• Syed B. (Siberian Federal University, 79 Svobodny pr., Krasnoyarsk, Russian Federation, Bionanotechnological Laboratory, Department of Studies in Microbiology, Manasagangotri, University of Mysore, Mysore, Karnataka, India)
• Nagendra Prasad M.N. (Department of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, JSS Technical Institutional Campus, Mysore, India)
• Mohan Kumar K. (Department of Chemistry, Madanapalle Institute of Technology & Science, Post Box No: 14, Kadiri Road, Angallu (V), Madanapalle, Chittoor District, Andhra Pradesh, India)
• Satish S. (Bionanotechnological Laboratory, Department of Studies in Microbiology, Manasagangotri, University of Mysore, Mysore, Karnataka, India)

• Scopus
• Web of Science Core Collection
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