Сибирский федеральный университет

Перевод названия: Anthocyanins of fetuses bilberry ( Vaccinium myrtillus L.) which growing in Krasnoyarsk krai

Тип публикации: статья из журнала

Год издания: 2015

Ключевые слова: Vaccinium myrtillus L, delphinidin-3-glucoside, ethyl hydroxide, anthocyanins, Hplc, дельфинидин-3-глюкозид, этиловый спирт, антоцианы, ВЭЖХ

Аннотация: Методом ВЭЖХ с УФ-детектированием определён антоциановый состав мороженых плодов черники обыкновенной ( Vaccinium myrtillus L.), собранных на территории Енисейского района Красноярского края. Отмечены условия для оптимального извлечения антоцианов этиловым спиртом разной концентрации. It is known that anthocyanins are effective antioxidants and neutralize free oxygen radicals; have decongestant effect and reduce the permeability of the capillaries; have a protective effect on blood vessels, reducing capillary fragility and have a positive effect on vascular lesions; contribute to the stabilization of the connective tissue, normalizing the outflow of intraocular fluid in glaucoma and intraocular pressure. There are numbers of papers dedicated to the study of anthocyanins bilberry. In the main papers used the methods TLC and electron spectroscopy methods are used. But there is not information about anthocyanins of bilberry growing in Krasnoyarsk krai. In this regard, the aim of this work was to find the optimal extraction conditions and determine the composition of anthocyanins in bilberry growing in Krasnoyarsk krai by HPLC. Anthocyanins composition of frozen fetuses bilberry ( Vaccinium myrtillus L.) which grows in Yenisei district of Krasnoyarsk krai was investigated by HPLC method with UV detection. There were prepared fifty ethanolic extracts of berries during the experiment, 5 of which infused in 40% ethanol containing 1% HCl at room temperature for 15, 30, 45, 60, 90 minutes at a ratio of feed: extractant 1:30. 45 extracts obtained by heating on a boiling water bath for 15, 30, 45, 60 and 90 minutes with the ratio of feed: extractant 1:30, 1:50 and 1:100. We used 40%, 60% and 95% ethanol with addition of c 1% HCl for extraction. There were found the conditions for optimal extraction of anthocyanins with different concentrations of ethanol (extraction on a boiling water bath for 15 minutes and 40% ethanol containing 1% HCl and ratio of raw materials: extractant 1:30). Maximum cumulative quantitative recovery of this method was 3764.69 ± 0.14. We quantified the content of anthocyanins based on the delphinidin-3-glucoside 9.85 ± 0.08%. Anthocyanin composition of bilberry berries growing in the Krasnoyarsk krai defined by HPLC using a UV detector.

Журнал: Вопросы биологической, медицинской и фармацевтической химии

Выпуск журнала: № 6

Номера страниц: 29-34

ISSN журнала: 15609596

Место издания: Москва

Издатель: Общество с ограниченной ответственностью Издательский дом Русский врач

• Полина С.А. (Сибирский федеральный университет)
• Хмарская Н.Е. (Сибирский федеральный университет)
• Ефремов А.А. (Сибирский федеральный университет)

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