Gelatin and Starch: What Better Stabilizes the Enzyme Activity? : научное издание | Научно-инновационный портал СФУ

Gelatin and Starch: What Better Stabilizes the Enzyme Activity? : научное издание

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1134/S0012496620020039

Ключевые слова: butyrylcholinesterase, enzyme stabilization, gelatin, luciferase, NAD(P)H:FMN oxidoreductase, starch, thermal inactivation of enzymes

Аннотация: The regularities of the functioning of a number of enzymes in a viscous environment created by natural polymers, starch and gelatin are examined. Based on the analysis of kinetic curves of thermal inactivation, mechanisms of thermal inactivation of enzymes in a viscous microenvironment are proposed. Using the example of butyrylcholinesterase, NAD(P)H:FMN oxidoreductase, and coupled system of the luminous bacteria (NAD(P)H:FMN oxidoreductase + luciferase), the conditions, under which starch and gelatin have a stabilizing effect on enzyme activity during storage and exposure to various physical and chemical environmental factors, were found. A significant increase in the stabilizing effect is achieved by eliminating water during drying the enzyme preparations immobilized in starch and gelatin polymer gels

Ссылки на полный текст

Издание

Журнал: Doklady Biological Sciences

Выпуск журнала: Vol. 491, Is. 1

Номера страниц: 43-46

ISSN журнала: 01124966

Издатель: Pleiades Publishing

Авторы

  • Esimbekova E.N. (Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)
  • Govorun A.E. (Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)
  • Lonshakova-Mukina V.I. (Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)
  • Kratasyuk V.A. (Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)

Вхождение в базы данных

Информация о публикациях загружается с сайта службы поддержки публикационной активности СФУ. Сообщите, если заметили неточности.

Вы можете отметить интересные фрагменты текста, которые будут доступны по уникальной ссылке в адресной строке браузера.