Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria

Тип публикации: статья из журнала

Год издания: 2011

Идентификатор DOI: 10.1134/S0003683811050152

Ключевые слова: Bacteria (microorganisms), Cupriavidus, Cupriavidus necator

Аннотация: Synthesis of 3- and 4-hydroxybutyrate copolymer (3HB-co-4HB), the most promising member of the biodegradable polyhydroxyalkanoate (PHA) family, has been studied. Cultivation conditions of naturally occurring strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus B10646 have been optimized to ensure efficient synthesis of the 3HB-co-4HB copolymer. A set of highly pure samples of the 3HB-co-4HB copolymer with 4HB content varying from 8.7 to 24.3 mol% has been obtained. Incorporation of 4HB into the copolymer was shown to cause a more pronounced decrease in polymer crystallinity than the incorporation of 3-hydroxyvalerate or 3-hydroxyhexanoate; samples with a degree of crystallinity below 30% have been obtained. The weight average molecular mass of the 3HB-co-4HB copolymers was shown to be independent on the monomer ratio and to vary broadly (from 540 to 1110 kDa).

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Издание

Журнал: APPLIED BIOCHEMISTRY AND MICROBIOLOGY

Выпуск журнала: Vol. 47, Is. 5

Номера страниц: 494-499

ISSN журнала: 00036838

Место издания: NEW YORK

Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER

Авторы

  • Volova T.G. (Siberian Federal University)
  • Zhila N.O. (Biophysics Institute, Siberian Branch,Russian Academy of Sciences)
  • Kalacheva G.S. (Biophysics Institute, Siberian Branch,Russian Academy of Sciences)
  • Sokolenko V.A. (Chemistry and Chemical Technology Institute, Siberian Branch,Russian Academy of Sciences)
  • Sinski E.J. (Massachusetts Institute of Technology)

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