Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoate monomer by Cupriavidus eutrophus B10646

Тип публикации: статья из журнала

Год издания: 2016

Идентификатор DOI: 10.1002/jctb.4592

Ключевые слова: wild-type strain Cupriavidus eutrophus, B10646, growth kinetics, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), physicochemical and mechanical properties, Growth kinetics, Physicochemical and mechanical properties, Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), Wild-type strain Cupriavidus eutrophus B10646, Biomaterials, Biomechanics, Chemical industry, Cultivation, Growth kinetics, Mechanical properties, Organic compounds, Polymers, Sodium, 3-Hydroxyhexanoate, Bacterial strains, Cultivation conditions, Kinetic properties, Physico-chemical and mechanical properties, Physiological range, Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), Wild-type strain, Strain, copolymer, glucose, hexanoic acid, nitrogen, sodium, Article, bacterial strain, biomass, Cupriavidus, Cupriavidus eutrophus, differential scanning calorimetry, enzyme substrate, kinetics, measurement accuracy, nonhuman, synthesis, thermal analysis, wild type

Аннотация: BACKGROUNDP(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). The most common approach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this study was to optimize the process of production of P(3HB-co-3HHx) copolymers containing high molar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophusB10646. RESULTSKinetic properties of C. eutrophusB10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophusB10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSIONFor the first time, cultivation conditions of Cupriavidus eutrophusB10646 enabled production of high biomass yields (5-6gL(-1)) and high content of the polymer (60-75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers. (c) 2014 Society of Chemical Industry

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Издание

Журнал: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Выпуск журнала: Vol. 91, Is. 2

Номера страниц: 416-425

ISSN журнала: 02682575

Место издания: HOBOKEN

Издатель: WILEY-BLACKWELL

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