A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs

Тип публикации: статья из журнала

Год издания: 2014

Идентификатор DOI: 10.1371/journal.pone.0087551

Ключевые слова: 3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer, 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer, copolymer, gamma butyrolactone, glucose, hexanoic acid, poly(3 hydroxybutyric acid), polyhydroxyalkanoic acid, polystyrene, propionic acid, unclassified drug, valeric acid, culture medium, hexanoic acid derivative, polymer, propionic acid derivative, valeric acid derivative, animal cell, article, bacterial growth, bacterium culture, cell adhesion, cell proliferation, crystal structure, culture optimization, Cupriavidus, Cupriavidus euthrophus, decomposition, elasticity, film, glucose utilization, kinetics, mechanics, melting point, mouse, nonhuman, nucleotide sequence, physical chemistry, polymerization, strength, synthesis, 3T3 cell line, animal, cell line, culture medium, Cupriavidus, fibroblast, growth, development and aging, metabolism, temperature, 4-Butyrolactone, Animals, Caproates, Cell Line, Culture Media, Cupriavidus, Fibroblasts, Glucose, Kinetics, Mice, NIH 3T3 Cells, Polymers, Propionates, Temperature, Valerates

Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

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Издание

Журнал: PLOS ONE

Выпуск журнала: Vol. 9, Is. 2

ISSN журнала: 19326203

Место издания: SAN FRANCISCO

Издатель: PUBLIC LIBRARY SCIENCE

Авторы

  • Volova T. (Siberian Federal University)
  • Kiselev E. (Institute of Biophysics of Siberian Branch of Russian Academy of Sciences)
  • Nikolaeva E. (Institute of Biophysics of Siberian Branch of Russian Academy of Sciences)
  • Sukovatiy A. (Institute of Biophysics of Siberian Branch of Russian Academy of Sciences)
  • Shishatskaya E. (Siberian Federal University)
  • Vinogradova O. (Siberian Federal University)
  • Chistyakov A. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science)

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