System peaks and optimization of anion separation in capillary electrophoresis with non-reversed electroosmotic flow

Тип публикации: статья из журнала

Год издания: 2012

Идентификатор DOI: 10.1134/S1061934812090080

Ключевые слова: capillary electrophoresis, system peaks, optimization of anion separation, non-reversed electroosmotic flow, indirect detection, Non-reversed electroos-motic flow, Analytes, Anion separations, Back ground electrolyte, Electroosmotic flow, Electropherograms, Hydrodynamic pressure, Optimal conditions, Peak area, Spectrophotometric detection, Algorithms, Chromates, Detectors, Electrolytes, Electroosmosis, Optimization, Negative ions

Аннотация: We studied system peaks present in the electropherograms obtained in the separation of anions by capillary electrophoresis with indirect spectrophotometric detection and cathode electroosmotic flow (EOF) with a chromate background electrolyte. The system peaks correspond to the zones with changed concentration of the background electrolyte; they formed when the zones of each analyte passed through the outlet of the capillary and then moved towards the EOF detector. It has been revealed that the height and area of the system peaks linearly depends on the concentration of the corresponding anion and the areas of the system peaks can achieve 10% of the anion peak area. An algorithm has been proposed for the determination of the optimal conditions for anion separation using hydrodynamic pressure for the regulation of the EOF flow rate. This algorithm prevents the overlapping of the anion and system peaks.

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Издание

Журнал: JOURNAL OF ANALYTICAL CHEMISTRY

Выпуск журнала: Vol. 67, Is. 9

Номера страниц: 783-789

ISSN журнала: 10619348

Место издания: NEW YORK

Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER

Авторы

  • Sursyakova V.V. (Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Krasnoyarsk 660049, Russia)
  • Kalyakin S.N. (Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Krasnoyarsk 660049, Russia)
  • Burmakina G.V. (Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Krasnoyarsk 660049, Russia; Siberian Fed Univ, Dept Chem & Met, Krasnoyarsk 660041, Russia)
  • Rubaylo A.I. (Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Krasnoyarsk 660049, Russia; Siberian Fed Univ, Dept Chem & Met, Krasnoyarsk 660041, Russia)

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