Practical enzymology course based on bioluminescence

Тип публикации: статья из журнала (материалы конференций, опубликованные в журналах)

Конференция: 10th International Symposium on Bioluminescence and Chemiluminescence; BOLOGNA, ITALY; BOLOGNA, ITALY

Год издания: 1999

Идентификатор DOI: 10.1002/(SICI)1522-7243(199907/08)14:4<189::AID-BIO527>3.0.CO;2-E

Ключевые слова: enzyme; science education; luciferase; bioluminescence, Bioluminescence, Enzyme, Luciferase, Science education, enzyme, immobilized enzyme, article, biochemistry, continuing education, education, human, laboratory personnel, luminescence, Biochemistry, Education, Continuing, Enzymes, Enzymes, Immobilized, Humans, Laboratory Personnel, Luminescence

Аннотация: We describe our experience with laboratory courses in enzymology based on the phenomenon of bioluminescence. The soluble and immobilized enzymes of luminous bacteria are used and the practical enzymological course consists of four main courses: (1) training in measuring the activities of soluble and immobilized enzymes; (2) the investigation of kinetic characteristics (kinetic constants) and enzyme-substrate and enzyme-inhibitor interactions in the bacterial bioluminescent reaction; (3) The testing of physico-chemical characteristics of enzymes (pH, temperature, ion strength, etc.); (4) the effect of inhibitors on enzymes. Training is possible in groups of about ten persons. Our practice work has been introduced in the biological, pedagogical and physical departments of Krasnoyarsk State University. Students of the pedagogical department have created a popular and interesting series of laboratory works for high school children aged 14-17 years. Copyright (C) 1999 John Wiley & Sons, Ltd.

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Выпуск журнала: Vol. 14, Is. 4

Номера страниц: 189-192

ISSN журнала: 15227235

Место издания: W SUSSEX



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