Antioxidant activity of humic substances via bioluminescent monitoring in vitro

Тип публикации: статья из журнала

Год издания: 2015

Идентификатор DOI: 10.1007/s10661-015-4304-1

Ключевые слова: Antioxidant activity, Oxidative toxicity, General toxicity, Humic substances, Bioassay, Bioluminescence, Antioxidant activity, Bioassay, Bioluminescence, General toxicity, Humic substances, Oxidative toxicity, Antioxidants, Bioassay, Bioluminescence, Detoxification, Phosphorescence, Plants (botany), Water pollution, Anti-oxidant activities, Antioxidant efficiencies, Antioxidant properties, Bioluminescent systems, Environmental and waste water, Humic substances, Oxidative toxicity, Remediation procedures, Toxicity, 1,4 benzoquinone, alkanal monooxygenase (FMN linked), flavine mononucleotide reductase, potassium ferricyanide, alkanol monooxygenase (FMN-linked), antioxidant, benzoquinone, benzoquinone derivative, humic substance, inorganic salt, luciferase, antioxidant, bioassay, bioluminescence, detoxification, enzyme, humic substance, pollution monitoring, toxicity, Aliivibrio fischeri, Article, bioluminescence, catalysis, controlled study, detoxification, humic substance, in vitro study, nonhuman, oxidative stress, Photobacterium leiognathi, water pollutant, analysis, environmental monitoring, humic substance, luminescence, metabolism, microbiology, oxidation reduction reaction, procedures, Bacteria (microorganisms), Antioxidants, Benzoquinones, Environmental Monitoring, FMN Reductase, Humic Substances, In Vitro Techniques, Luciferases, Luminescent Measurements, Oxidation-Reduction, Salts, Soil Microbiology

Аннотация: This work considers antioxidant properties of natural detoxifying agents-humic substances (HS) in solutions of model inorganic and organic compounds of oxidative nature-complex salt K-3[Fe(CN)(6)] and 1,4-benzoquinone. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P) H:FMN-oxidoreductase and bacterial luciferase was used as a bioassay in vitro to monitor toxicity of the oxidizer solutions. Toxicities of general and oxidative types were evaluated using bioluminescent kinetic parameters-bioluminescence intensity and induction period, respectively. Antioxidant activity of HS was attributed to their ability to decrease both general and oxidative toxicities; the HS antioxidant efficiency was characterized with detoxification coefficients D-GT and D-OxT, respectively. Dependencies of D-GT and D-OxT on HS concentration and time of preliminary incubation of the oxidizers with HS were demonstrated. The optimal conditions for detoxification of the oxidizers were >20-min incubation time and 0.5x10(-4) to 2x10(-4) M of HS concentration. The present study promotes application of the enzymatic luminescent bioassay to monitor toxicity of pollutants of oxidative nature in environmental and waste waters in remediation procedures.

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Издание

Журнал: ENVIRONMENTAL MONITORING AND ASSESSMENT

Выпуск журнала: Vol. 187, Is. 3

ISSN журнала: 01676369

Место издания: DORDRECHT

Издатель: SPRINGER

Авторы

  • Tarasova A.S. (Institute of Biophysics SB RAS)
  • Kudryasheva N.S. (Institute of Biophysics SB RAS)
  • Stom D.I. (Irkutsk State University)

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