Тип публикации: статья из журнала
Год издания: 2019
Идентификатор DOI: 10.3390/plants8110461
Ключевые слова: Dipterocarpoideae, Genetic distance, MatK, Rbcl, Reference DNA library, TrnL-F, Tropical tree diversity
Аннотация: DNA barcoding has been used as a universal tool for phylogenetic inferences and diversity assessments, especially in poorly studied species and regions. The aim of this study was to contrast morphological taxonomy and DNA barcoding, using the three frequently used markers matK, rbcL, and trnL-F, to assess the effciency of DNA barcoding in the identification of dipterocarps in Sumatra, Indonesia. The chloroplast gene matK was the most polymorphic among these three markers with an average interspecific genetic distance of 0.020. The results of the molecular data were mostly in agreement with the morphological identification for the clades of Anthoshorea, Hopea, Richetia, Parashorea, and Anisoptera, nonetheless these markers were ineffcient to resolve the relationships within the Rubroshorea group. The maximum likelihood and Bayesian inference phylogenies identified Shorea as a paraphyletic genus, Anthoshorea appeared as sister to Hopea, and Richetia was sister to Parashorea. A better discriminatory power among dipterocarp species provided by matK and observed in our study suggests that this marker has a higher evolutionary rate than the other two markers tested. However, a combination of several different barcoding markers is essential for reliable identification of the species at a lower taxonomic level. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Выпуск журнала: Vol. 8, Is. 11
Номера страниц: 461
ISSN журнала: 22237747
Издатель: MDPI AG
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