Integrating DNA barcoding and traditional taxonomy for the identification of dipterocarps in remnant lowland forests of sumatra

Тип публикации: статья из журнала

Год издания: 2019

Идентификатор DOI: 10.3390/plants8110461

Ключевые слова: Dipterocarpoideae, Genetic distance, MatK, Rbcl, Reference DNA library, TrnL-F, Tropical tree diversity

Аннотация: DNA barcoding has been used as a universal tool for phylogenetic inferences and diversity assessments, especially in poorly studied species and regions. The aim of this study was to contrast morphological taxonomy and DNA barcoding, using the three frequently used markers matK, rbcL, and trnL-F, to assess the effciency of DNA barcoding in the identification of dipterocarps in Sumatra, Indonesia. The chloroplast gene matK was the most polymorphic among these three markers with an average interspecific genetic distance of 0.020. The results of the molecular data were mostly in agreement with the morphological identification for the clades of Anthoshorea, Hopea, Richetia, Parashorea, and Anisoptera, nonetheless these markers were ineffcient to resolve the relationships within the Rubroshorea group. The maximum likelihood and Bayesian inference phylogenies identified Shorea as a paraphyletic genus, Anthoshorea appeared as sister to Hopea, and Richetia was sister to Parashorea. A better discriminatory power among dipterocarp species provided by matK and observed in our study suggests that this marker has a higher evolutionary rate than the other two markers tested. However, a combination of several different barcoding markers is essential for reliable identification of the species at a lower taxonomic level. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

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Издание

Журнал: Plants

Выпуск журнала: Vol. 8, Is. 11

Номера страниц: 461

ISSN журнала: 22237747

Издатель: MDPI AG

Авторы

  • Moura Carina Carneiro de Melo (Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany)
  • Brambach Fabian (Univ Gottingen, Biodivers Macroecol & Biogeog, Busgenweg 1, D-37077 Gottingen, Germany)
  • Bado Kevin Jair Hernandez (Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany)
  • Krutovsky Konstantin (Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany; Univ Gottingen, Ctr Integrated Breeding Res, Albrecht Thaer Weg 3, D-37075 Gottingen, Germany; Russian Acad Sci, Lab Populat Genet, NI Vavilov Inst Gen Genet, 3 Gubkin Str, Moscow 119333, Russia; Siberian Fed Univ, Genome Res & Educ Ctr, Inst Fundamental Biol & Biotechnol, Lab Forest Genom, 50a-2 Akademgorodok, Krasnoyarsk 660036, Russia)
  • Kreft Holger (Univ Gottingen, Biodivers Macroecol & Biogeog, Busgenweg 1, D-37077 Gottingen, Germany)
  • Tjitrosoedirdjo Sri Sudarmiyati (Southeast Asian Reg Ctr Trop Biol SEAMEO BIOTROP, Jalan Raya Tajur Km 6, Bogor 16144, Indonesia)
  • Siregar Iskandar Z. (Bogor Agr Univ, Fac Forestry, Dept Silviculture, Dramaga Campus, Bogor 16680, IndonesiaArticle)
  • Gailing Oliver (Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany; Univ Gottingen, Ctr Integrated Breeding Res, Albrecht Thaer Weg 3, D-37075 Gottingen, Germany)

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