Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1002/elps.202000189

Ключевые слова: capillary electrophoresis, drug delivery, host&#8211, guest complexes, pentacyclic lupane triterpenoids, stability constant, host–guest complexes

Аннотация: The complexation of ester betulin derivatives with (2-hydroxypropyl)-beta-cyclodextrin (HP-beta-CD) was studied by mobility shift affinity CE. Electrophoretic mobility for triangular peaks was calculated using the parameter a(1) of the Haarhoff-Van der Linde function instead of the peak top time. Dependences of the viscosity corrected electrophoretic mobility on HP-beta-CD concentration were not described on the basis of only complexes with 1:1 stoichiometry due to the fact that these binding curves did not reach a plateau. However, the dependences were well described taking into account both 1:1 and 1:2 complexes. The presence of higher order equilibria was also revealed by x-reciprocal plots. The values of apparent binding constant logarithm, obtained for the first time, for 1:1 and 1:2 HP-beta-CD complexes of betulin 3,28-diphthalate and betulin 3,28-disuccinate with 95% confidence interval limits in brackets are the same within error and are equal to 4.85 (4.73-4.95), 8.56 (7.75-8.82), 4.92 (4.86-4.97), and 8.54 (8.23-8.72) at 25 degrees C, respectively. These values for 1:1 and 1:2 HP-beta-CD complexes of betulin 3,28-disulfate at 25 degrees C are 4.61 (4.57-4.64) and 7.11 (6.57-7.34), respectively. The binding constants for betulin 3,28-disulfate agree with the previously obtained results from the separation in the thermostatted capillary segment.

Журнал: ELECTROPHORESIS

Номера страниц: 700-707

ISSN журнала: 01730835

Место издания: HOBOKEN

Издатель: WILEY

• Sursyakova Viktoria V. (Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia)
• Levdansky Vladimir A. (Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia)
• Rubaylo Anatoly I. (Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Sch Nonferrous Met & Mat Sci, Krasnoyarsk, Russia; Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk, Russia)

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