Study of carboxyhemoglobin photodissociation with laser flash-photolysis

Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций

Конференция: 2003 Saratov Fall Meeting; Saratov, RUSSIA; Saratov, RUSSIA

Год издания: 2004

Идентификатор DOI: 10.1117/12.578359

Ключевые слова: carboxyhemoglobin; carbon monoxide; efficiency of photodissociation; laser photolysis

Аннотация: Assessment of the carboxyhemoglobin photodissociation has been performed under the native conditions. This investigation has a great importance for the development and creation of completely new approach for the treatment of carbon monoxide poisoning based on the photoinduced dissociation of carboxyhemoglobin. Photodissociation was registered on the experimental setup with crossing laser beams where pulsed Nd:YAG laser at the second harmonics wavelength (lambda=532 nm) was used as a source of photolyzing radiation. Buffered solutions of whole human peripheral blood (PBS, pH=7.4) and diluted hemolized human peripheral blood were used. We found optimal parameters for the registration of the photodissociation such as using of buffered solutions of the whole human peripheral blood with the concentration of carboxyhemoglobin around 50%, detection of dissociation of carboxyhemoglobin at the maximum of absorption within the Soret's band (435 nm). Dependence of photodissociation efficiency on the concentration of the complex in the tested solutions, as well as on the photolysis radiation intensity in both types of solutions was proved.

Ссылки на полный текст

Издание

Журнал: SARATOV FALL MEETING 2003: OPTICAL TECHNOLOGIES IN BIOPHYSICS AND MEDICINE V

Выпуск журнала: Vol. 5474

Номера страниц: 88-

ISSN журнала: 0277786X

Место издания: BELLINGHAM

Издатель: SPIE-INT SOC OPTICAL ENGINEERING

Авторы

Вхождение в базы данных

Информация о публикациях загружается с сайта службы поддержки публикационной активности СФУ. Сообщите, если заметили неточности.

Вы можете отметить интересные фрагменты текста, которые будут доступны по уникальной ссылке в адресной строке браузера.