Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.3390/genes11010011

Адрес в архиве электронных ресурсов СФУ: https://elib.sfu-kras.ru/handle/2311/137960

Ключевые слова: Chitinase, Exons, Flavonoid biosynthesis pathway, Fragaria, Introns, Microsatellites, Polymorphism, Rubus, Transcription factor genes, Transferability

Аннотация: Strawberry (Fragaria) and raspberry (Rubus) are very popular crops, and improving their nutritional quality and disease resistance are important tasks in their breeding programs that are becoming increasingly based on use of functional DNA markers. We identified 118 microsatellite (simple sequence repeat—SSR) loci in the nucleotide sequences of flavonoid biosynthesis and pathogenesis-related genes and developed 24 SSR markers representing some of these structural and regulatory genes. These markers were used to assess the genetic diversity of 48 Fragaria and Rubus specimens, including wild species and rare cultivars, which differ in berry color, ploidy, and origin. We have demonstrated that a high proportion of the developed markers are transferable within and between Fragaria and Rubus genera and are polymorphic. Transferability and polymorphism of the SSR markers depended on location of their polymerase chain reaction (PCR) primer annealing sites and microsatellite loci in genes, respectively. High polymorphism of the SSR markers in regulatory flavonoid biosynthesis genes suggests their allelic variability that can be potentially associated with differences in flavonoid accumulation and composition. This set of SSR markers may be a useful molecular tool in strawberry and raspberry breeding programs for improvement anthocyanin related traits. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Журнал: Genes

Выпуск журнала: Vol. 11, Is. 1

Номера страниц: 11

ISSN журнала: 20734425

Издатель: MDPI AG

• Lebedev Vadim G. (Pushchino State Inst Nat Sci, Prospekt Nauki 3, Pushchino 142290, Russia; Russian Acad Sci, Branch Shemyakin Ovchinnikov Inst Bioorgan Chem, Prospekt Nauki 6, Pushchino 142290, Russia)
• Subbotina Natalya M. (Pushchino State Inst Nat Sci, Prospekt Nauki 3, Pushchino 142290, Russia; Russian Acad Sci, Branch Shemyakin Ovchinnikov Inst Bioorgan Chem, Prospekt Nauki 6, Pushchino 142290, Russia)
• Maluchenko Oleg P. (All Russian Res Inst Agr Biotechnol, Timiriazevskaya Str 42, Moscow 127550, Russia)
• Lebedeva Tatyana N. (Russian Acad Sci, Inst Physicochem & Biol Problems Soil Sci, Inst Skaya Str 2, Pushchino 142290, Russia)
• Krutovsky Konstantin V. (Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany; Georg August Univ Gottingen, Ctr Integrated Breeding Res, Albrecht Thaer Weg 3, D-37075 Gottingen, Germany; Russian Acad Sci, NI Vavilov Inst Gen Genet, Lab Populat Genet, Gubkin Str 3, Moscow 119333, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Genome Res & Educ Ctr, Lab Forest Genom, Krasnoyarsk 660036, Russia)
• Shestibratov Konstantin A. (Russian Acad Sci, Branch Shemyakin Ovchinnikov Inst Bioorgan Chem, Prospekt Nauki 6, Pushchino 142290, Russia)

• Scopus
• Web of Science Core Collection