Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций
Конференция: European Symposium on Biopolymers; Kusadasi, TURKEY; Kusadasi, TURKEY
Год издания: 2008
Идентификатор DOI: 10.1002/masy.200850901
Ключевые слова: autotrophic and mixotrophic growth, multi-component polyhydroxyalkanoates, Wautersia eutropha, Bacteriology, Biochemical engineering, Biochemistry, Biopolymers, Biotechnology, Carboxylic acids, Fatty acids, Ketones, Monomers, Polymers, Carbon chains, Carbon nutritions, Even numbers, Growth conditions, Hydroxybutyrate, Hydroxyvalerate, Mixotrophic, Odd numbers, Physico-chemical properties, Polyhydroxyalkanoates, Synthesis of, Wild types, Acids
Аннотация: The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild type strains (H16 and 85786), it has been first found that under mixotrophic growth conditions -CO2+ co-substrate (alkanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chain-length monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate as major monomers and hydroxyhexanoate, hydroxyheptanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs were synthesized and their physicochemical properties examined.
Издание
Журнал: MACROMOLECULAR SYMPOSIA
Выпуск журнала: Vol. 269
Номера страниц: 1-7
ISSN журнала: 10221360
Место издания: WEINHEIM
Издатель: WILEY-V C H VERLAG GMBH
Персоны
- Volova T.G. (Siberian Federal University)
- Kalacheva G.S. (Institute of Biophysics,SB,RAS)
- Steinbuchel A. (Institute of Molecular Microbiology and Biotechnology)
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