Development of Bacteriostatic DNA Aptamers for Salmonella

Тип публикации: статья из журнала

Год издания: 2013

Идентификатор DOI: 10.1021/jm301856j

Ключевые слова: amikacin, ampicillin, antibiotic agent, aptamer, bacterial DNA, chloramphenicol, streptomycin, tetracycline, antibacterial activity, antibiotic resistance, article, bacterial growth, bactericidal activity, bacteriostasis, bacterium culture, cell membrane depolarization, dissociation constant, DNA sequence, drug binding site, drug DNA binding, human, membrane potential, nonhuman, Salmonella enteritidis, Salmonella typhimurium, serotype, Anti-Bacterial Agents, Aptamers, Nucleotide, Base Sequence, Drug Resistance, Multiple, Bacterial, Molecular Sequence Data, Salmonella enteritidis, Salmonella typhimurium, SELEX Aptamer Technique, Structure-Activity Relationship

Аннотация: Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Aptamers have grown in popularity since their discovery, and their properties make them attractive candidates for therapeutic use. In this work, we describe the selection of highly specific DNA aptamers to S. enteritidis and S. typhimurium. To evolve species specific aptamers, twelve rounds of selection to live S. enteritidis and S. typhimurium were performed, alternating with a negative selection against a mixture of related pathogens. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential.

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Издание

Журнал: JOURNAL OF MEDICINAL CHEMISTRY

Выпуск журнала: Vol. 56, Is. 4

Номера страниц: 1564-1572

ISSN журнала: 00222623

Место издания: WASHINGTON

Издатель: AMER CHEMICAL SOC

Авторы

  • Kolovskaya Olga S. (Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia)
  • Savitskaya Anna G. (Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia)
  • Zamay Tatiana N. (Krasnoyarsk State Med Univ, Dept Biol Chem, Krasnoyarsk 660022, Russia)
  • Reshetneva Irina T. (Krasnoyarsk State Med Univ, Dept Microbiol, Krasnoyarsk 660022, Russia)
  • Zamay Galina S. (Krasnoyarsk State Med Univ, Dept Biol Chem, Krasnoyarsk 660022, Russia)
  • Erkaev Evgeny N. (Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia)
  • Wang Xiaoyan (Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada)
  • Wehbe Mohamed (Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada)
  • Salmina Alla B. (Krasnoyarsk State Med Univ, Dept Biol Chem, Krasnoyarsk 660022, Russia)
  • Perianova Olga V. (Krasnoyarsk State Med Univ, Dept Microbiol, Krasnoyarsk 660022, Russia)
  • Zubkova Olga A. (Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia)
  • Spivak Ekaterina A. (Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia)
  • Mezko Vasily S. (Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia)
  • Glazyrin Yury E. (Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia)
  • Titova Nadezhda M. (Siberian Fed Univ, Dept Med Biol, Krasnoyarsk 660041, Russia)
  • Berezovski Maxim V. (Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada)
  • Zamay Anna S. (Krasnoyarsk State Med Univ, Dept Biol Chem, Krasnoyarsk 660022, Russia)

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