Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2014

Идентификатор DOI: 10.1007/s00216-014-7656-4

Ключевые слова: Bioluminescence, Luciferase, Photoprotein, Coelenterazine, Calcium, Phosphorescence, Plants (botany), Tissue, Aequorea victoria, Deep tissue imaging, Electron-donating group, Intracellular calcium concentration, Photoproteins, Tissue penetrations, Tissue engineering, Animalia, Coelenterata, Obelia longissima, aequorin, imidazole derivative, luminescent agent, obelin, pyrazine derivative, animal, chemistry, CHO cell line, Cricetulus, devices, Hydrozoa, luminescence, molecular imaging, procedures, Animals, CHO Cells, Imidazoles, Luminescent Agents, Luminescent Measurements, Luminescent Proteins, Pyrazines

Аннотация: The main analytical use of Ca2+-regulated photoproteins from luminous coelenterates is for real-time non-invasive visualization of intracellular calcium concentration ([Ca2+](i)) dynamics in cells and whole organisms. A limitation of this approach for in vivo deep tissue imaging is the fact that blue light emitted by the photoprotein is highly absorbed by tissue. Seven novel coelenterazine analogues were synthesized and their effects on the bioluminescent properties of recombinant obelin from Obelia longissima and aequorin from Aequorea victoria were evaluated. Only analogues having electron-donating groups (m-OCH3 and m-OH) on the C6 phenol moiety or an extended resonance system at the C8 position (1-naphthyl and alpha-styryl analogues) showed a significant red shift of light emission. Of these, only the alpha-styryl analogue displayed a sufficiently high light intensity to allow eventual tissue penetration. The possible suitability of this compound for in vivo assays was corroborated by studies with aequorin which allowed the monitoring of [Ca2+](i) dynamics in cultured CHO cells and in hippocampal brain slices. Thus, the alpha-styryl coelenterazine analogue might be potentially useful for non-invasive, in vivo bioluminescence imaging in deep tissues of small animals.

Журнал: ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Выпуск журнала: Vol. 406, Is. 11

Номера страниц: 2695-2707

ISSN журнала: 16182642

Место издания: HEIDELBERG

Издатель: SPRINGER HEIDELBERG

• Gealageas Ronan (Ctr Natl Rech Sci, Inst Chim Subst Nat, UPR 2301, F-91198 Gif Sur Yvette, France)
• Malikova Natalia P. (Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia)
• Picaud Sandrine (Ctr Natl Rech Sci, Inst Neurosci Alfred Fessard, UPR 3294, F-91198 Gif Sur Yvette, France)
• Borgdorff Aren J. (Ctr Natl Rech Sci, Inst Neurosci Alfred Fessard, UPR 3294, F-91198 Gif Sur Yvette, France)
• Burakova Ludmila P. (Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia)
• Brulet Philippe (Ctr Natl Rech Sci, Inst Neurosci Alfred Fessard, UPR 3294, F-91198 Gif Sur Yvette, France)
• Vysotski Eugene S. (Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia)
• Dodd Robert H. (Ctr Natl Rech Sci, Inst Chim Subst Nat, UPR 2301, F-91198 Gif Sur Yvette, France)

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