Bioluminescent signal system: bioluminescence immunoassay of pathogenic organisms

Тип публикации: статья из журнала

Год издания: 2007

Идентификатор DOI: 10.1002/bio.952

Ключевые слова: obelin; bioluminescence immunoassay; infective agents

Аннотация: The Ca2+-regulated photoprotein obelin has been examined as a label for bioluminescence immunoassay of infective agents. The hepatitis B virus (HbsAg) and the bacteria Escherichia coli and Shigella sonnei lipopolysaccharide (LPS) were chosen as model antigens. Chemically synthesized obelin-corresponding antibody conjugates were used in a solid-phase microplate immunoassay. The sensitivities achieved by the assay were 0.25 ng/mL for S. sonnei LPS and 0.375 ng/mL for HbsAg. A novel, filter-based immunoassay to determine bacterial admixtures in the environment was proposed. The NanoCeram filters were effectively applied to 'trap' and pre-concentrate pathogens from samples under study for the purposes of further detection and measurement of the absorbed material by bioluminescence immunoassay. Copyright (C) 2007 John Wiley & Sons, Ltd.

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Издание

Журнал: LUMINESCENCE

Выпуск журнала: Vol. 22, Is. 3

Номера страниц: 215-220

ISSN журнала: 15227235

Место издания: CHICHESTER

Издатель: JOHN WILEY & SONS LTD

Авторы

  • Frank L. (Institute of Biophysics,Russian Academy of Sciences Siberian Branch)
  • Markova S. (Institute of Biophysics,Russian Academy of Sciences Siberian Branch)
  • Vysotski E. (Institute of Biophysics,Russian Academy of Sciences Siberian Branch)
  • Gitelson I. (Institute of Biophysics,Russian Academy of Sciences Siberian Branch)
  • Remmel N. (Krasnoyarsk State University)

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