Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2007

Идентификатор DOI: 10.1134/S0026261707060082

Ключевые слова: Wautersia eutropha; autotrophic and mixotrophic growth; multicomponent polyhydroxyalkanoates

Аннотация: The effect of carbon supply on polyhydroxyalkanoate (PHA) synthesis by bacteria Wautersia eutropha was studied. Synthesis of multicomponent PHA composed of short-and long-chain monomers (C-4-C-8) by two natural strains (H16 and B5786) under mixotrophic conditions (CO2 + alkanoic acids as cosubstrates) was demonstrated for the first time. The PHA composition was shown to be dependent on the cosubstrate type. In the presence of odd fatty acids, four-and five-component polymers were synthesized; hydroxybutyrate, hydroxyvalerate, and hydroxyheptanoate were the major monomers, while hydroxyhexanoate and hydroxyoctanoate were minor. In the presence of even fatty acids, PHA contained not only the corresponding molecules (hydroxyhexanoate and hydroxyoctanoate), but also hydroxyvalerate; synthesis of four-component PHA which contain mainly hydroxybutyrate and hydroxyhexanoate (up to 18 mol %) is therefore possible. A series of four-and five-component PHA was synthesized and their physicochemical characteristics were determined.

Журнал: MICROBIOLOGY

Выпуск журнала: Vol. 76, Is. 6

Номера страниц: 704-711

ISSN журнала: 00262617

Место издания: NEW YORK

Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER

• Volova T.G. (Institute of Biophysics,Siberian Branch,Russian Academy of Sciences)
• Kalacheva G.S. (Krasnoyarsk State University)
• Kozhevnikov I.V. (Krasnoyarsk State University)
• Steinbuchel A. (Institute of Molecular Biology and Biotechnology)

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